Evidence map›Paper›PMID 39003263›Full record

ArticleNature communications2024

Engineering programmable material-to-cell pathways via synthetic notch receptors to spatially control differentiation in multicellular constructs.

Mher Garibyan, Tyler Hoffman, Thijs Makaske, Stephanie K Do, Yifan Wu, Brian A Williams, Alexander R March, Nathan Cho, Nicolas Pedroncelli, Ricardo Espinosa Lima and 8 more

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

18 authors.

Mher Garibyan *Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of USC, University of Southern California, Los Angeles, CA, USA.ORCID 0009-0002-4168-0375
Tyler Hoffman *Department of Bioengineering, University of California Los Angeles, Los Angeles, CA, USA.
Thijs MakaskeDepartment of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of USC, University of Southern California, Los Angeles, CA, USA.
Stephanie K DoAlfred E. Mann Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA.ORCID 0009-0007-6101-0068
Yifan WuDepartment of Bioengineering, University of California Los Angeles, Los Angeles, CA, USA.
Brian A WilliamsDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Alexander R MarchDepartment of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of USC, University of Southern California, Los Angeles, CA, USA.ORCID 0000-0001-7495-1739
Nathan ChoAlfred E. Mann Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA.
Nicolas PedroncelliDepartment of Bioengineering, University of California Los Angeles, Los Angeles, CA, USA.
Ricardo Espinosa LimaDepartment of Bioengineering, University of California Los Angeles, Los Angeles, CA, USA.
Jennifer SotoDepartment of Bioengineering, University of California Los Angeles, Los Angeles, CA, USA.
Brooke JacksonDepartment of Bioengineering, University of California Los Angeles, Los Angeles, CA, USA.
Jeffrey W SantosoAlfred E. Mann Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA.ORCID 0000-0003-3321-9829
Ali KhademhosseiniDepartment of Bioengineering, University of California Los Angeles, Los Angeles, CA, USA.
Matt ThomsonDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.ORCID 0000-0003-1021-1234
Song LiDepartment of Bioengineering, University of California Los Angeles, Los Angeles, CA, USA.ORCID 0000-0002-4760-8828
Megan L McCainDepartment of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of USC, University of Southern California, Los Angeles, CA, USA. mlmccain@usc.edu.ORCID 0000-0003-1908-6783
Leonardo MorsutDepartment of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of USC, University of Southern California, Los Angeles, CA, USA. morsut@usc.edu.ORCID 0000-0001-7049-3478

Funding

Vascular Biology Training GrantT32HL069766 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LUSIS, ALDONS JAKE · 2002 to 2022
$7.4M
Systems Genetics Dissection of Non-alcoholic SteatohepatitisR01DK117850 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Aldons Jake Lusis · 2019 to 2026
$4.6M
Multimodal wireless electrical stimulation for tissue regenerationR01NS126918 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Song Li, John Rogers · 2022 to 2026
$2.4M
Synthetic development: dissection of morphogenetic programs via reconstructive and perturbative approachesR35GM138256 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI MORSUT, LEONARDO · 2020 to 2024
$2.1M
Regulation of cell reprogramming by matrix stiffnessR01GM143485 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LI, SONG · 2021 to 2024
$1.3M
NHLBI NIH HHS T32 HL069766NIDDK NIH HHS R01 DK117850NIGMS NIH HHS R01 GM143485NIGMS NIH HHS R35 GM138256NINDS NIH HHS R01 NS126918NSF | ENG/OAD | Division of Chemical, Bioengineering, Environmental, and Transport Systems (CBET) 2034495NSF | ENG/OAD | Division of Chemical, Bioengineering, Environmental, and Transport Systems (CBET) 2145528U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) GM143485U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) R35GM138256Wellcome Trust
6 · The paper itself

Abstract

Synthetic Notch (synNotch) receptors are genetically encoded, modular synthetic receptors that enable mammalian cells to detect environmental signals and respond by activating user-prescribed transcriptional programs. Although some materials have been modified to present synNotch ligands with coarse spatial control, applications in tissue engineering generally require extracellular matrix (ECM)-derived scaffolds and/or finer spatial positioning of multiple ligands. Thus, we develop here a suite of materials that activate synNotch receptors for generalizable engineering of material-to-cell signaling. We genetically and chemically fuse functional synNotch ligands to ECM proteins and ECM-derived materials. We also generate tissues with microscale precision over four distinct reporter phenotypes by culturing cells with two orthogonal synNotch programs on surfaces microcontact-printed with two synNotch ligands. Finally, we showcase applications in tissue engineering by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined micropatterns. These technologies provide avenues for spatially controlling cellular phenotypes in mammalian tissues.

Indexed as

Cell DifferentiationReceptors, NotchSignal TransductionTissue EngineeringAnimalsEndothelial CellsExtracellular MatrixExtracellular Matrix ProteinsFibroblastsHEK293 CellsHumansLigandsMiceMuscle, SkeletalTissue ScaffoldsExtracellular Matrix ProteinsLigandsReceptors, Notch

Identifiers

PMID39003263
PMCPMC11246427

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.