Evidence map›Paper›PMID 39002874›Full record

ArticleInfection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases2024

Comprehensive genomics reveals novel sequence types of multidrug resistant Klebsiella oxytoca with uncharacterized capsular polysaccharide K- and lipopolysaccharide O-antigen loci from the National Hospital of Uganda.

Reuben S Maghembe, Maximilian A K Magulye, Abdalah Makaranga, Edward Moto, Simon Sekyanzi, Savannah Mwesigwa, Eric Katagirya

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Article in Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

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0cells of the map it votes in
4citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Reuben S MaghembeDepartment of Microbiology and Immunology, Faculty of Biomedical Sciences, Kampala International University-Western Campus (KIU-WC), Ishaka, Uganda; Department of Immunology and Molecular Biology, School of Biomedical Sciences, Makerere University, P. O. Box 7072, Kampala, Uganda; Biological and Marine Sciences Unit, Faculty of Science, Marian University College, P. O. Box 47, Bagamoyo, Tanzania; Department of Biomedial Sciences, Didia Education and Health Organization (DEHO), P. O. Box 113, Shinyanga, Tanzania. Electronic address: rmaghembe@gmail.com.
Maximilian A K MagulyeDepartment of Immunology and Molecular Biology, School of Biomedical Sciences, Makerere University, P. O. Box 7072, Kampala, Uganda; Department of Biomedial Sciences, Didia Education and Health Organization (DEHO), P. O. Box 113, Shinyanga, Tanzania.
Abdalah MakarangaBiological and Marine Sciences Unit, Faculty of Science, Marian University College, P. O. Box 47, Bagamoyo, Tanzania.
Edward MotoDepartment of Biology, College of Natural and Mathematical Sciences, University of Dodoma, Dodoma, Tanzania.
Simon SekyanziDepartment of Medical Microbiology, 2(nd) Floor Pathology BLDG, College of Health Sciences, Makerere University, Upper Mulago Hill Road, P.O. Box 7072, Kampala, Uganda.
Savannah MwesigwaDepartment of Immunology and Molecular Biology, School of Biomedical Sciences, Makerere University, P. O. Box 7072, Kampala, Uganda.
Eric KatagiryaDepartment of Immunology and Molecular Biology, School of Biomedical Sciences, Makerere University, P. O. Box 7072, Kampala, Uganda.

Funding

Microbiology and Immunology Training for HIV and HIV-Related Research in Uganda (MITHU)D43TW010319 · FIC · CASE WESTERN RESERVE UNIVERSITY · PI BOOM, W. HENRY, JOLOBA, MOSES LUTAAKOME · 2016 to 2025
$3.1M
FIC NIH HHS D43 TW010319
6 · The paper itself

Abstract

The Klebsiella oxytoca complex comprises diverse opportunistic bacterial pathogens associated with hospital and community-acquired infections with growing alarming antimicrobial resistance. We aimed to uncover the genomic features underlying the virulence and antimicrobial resistance of isolates from Mulago National Hospital in Uganda. We coupled whole genome sequencing with Pathogenwatch multilocus sequence typing (MLST) and downstream bioinformatic analysis to delineate sequence types (STs) capsular polysaccharide K- and O-antigen loci, along with antimicrobial resistance (AMR) profiles of eight clinical isolates from the National Referral Hospital of Uganda. Our findings revealed that only two isolates (RSM6774 and RSM7756) possess a known capsular polysaccharide K-locus (KL74). The rest carry various unknown K-loci (KL115, KL128, KLI52, KL161 and KLI63). We also found that two isolates possess unknown loci for the lipopolysaccharide O-antigen (O1/O2v1 type OL104 and unknown O1). The rest possess known O1 and O3 serotypes. From MLST, we found four novel sequence types (STs), carrying novel alleles for the housekeeping genes glyceraldehyde-6-phosphate dehydrogenase A (gapA), glucose-6-phosphate isomerase (pgi), and RNA polymerase subunit beta (rpoB). Our AMR analysis revealed that all the isolates are resistant to ampicillin and ceftriaxone, with varied resistance to other antibiotics, but all carry genes for extended-spectrum beta-lactamases (ESBLs). Notably, one strain (RSM7756) possesses outstanding chromosomal and plasmid-encoded AMR to beta-lactams, cephalosporins, fluoroquinolones and methoprims. Conclusively, clinical samples from Mulago National Referral Hospital harbor novel STs and multidrug resistant K. oxytoca strains, with significant public health importance, which could have been underrated.

Indexed as

Drug Resistance, Multiple, BacterialKlebsiella oxytocaO AntigensAnti-Bacterial AgentsGenomicsHumansKlebsiella InfectionsMultilocus Sequence TypingPolysaccharides, BacterialUgandaWhole Genome SequencingAnti-Bacterial AgentsO AntigensPolysaccharides, BacterialAntimicrobial resistanceCapsular polysaccharidesGenomicsKlebsiella oxytocaMultilocus sequence typing (MLST)O-antigensVirulence factors

Identifiers

PMID39002874
PMCPMC11415533

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.