Evidence map›Paper›PMID 39002127›Full record

ArticleCell reports2024

Multi-step regulation of microRNA expression and secretion into small extracellular vesicles by insulin.

Marsel Lino, Ruben Garcia-Martin, Vitor Rosetto Muñoz, Gabriel Palermo Ruiz, Allah Nawaz, Bruna Brasil Brandão, Jonathan Dreyfus, Hui Pan, C Ronald Kahn

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Review
  6. Review
  7. Insulin Resistance and Inflammation.International journal of molecular sciences · 2026
    Review
  8. Review
  9. Review
  10. Review
  11. Review
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Marsel LinoJoslin Diabetes Center, Harvard Medical School, Harvard University, Boston, MA, USA; Harvard Medical School, Harvard University, Boston, MA, USA.
Ruben Garcia-MartinJoslin Diabetes Center, Harvard Medical School, Harvard University, Boston, MA, USA; Harvard Medical School, Harvard University, Boston, MA, USA.
Vitor Rosetto MuñozJoslin Diabetes Center, Harvard Medical School, Harvard University, Boston, MA, USA; Harvard Medical School, Harvard University, Boston, MA, USA.
Gabriel Palermo RuizJoslin Diabetes Center, Harvard Medical School, Harvard University, Boston, MA, USA.
Allah NawazJoslin Diabetes Center, Harvard Medical School, Harvard University, Boston, MA, USA; Harvard Medical School, Harvard University, Boston, MA, USA.
Bruna Brasil BrandãoJoslin Diabetes Center, Harvard Medical School, Harvard University, Boston, MA, USA; Harvard Medical School, Harvard University, Boston, MA, USA.
Jonathan DreyfusJoslin Diabetes Center, Harvard Medical School, Harvard University, Boston, MA, USA.
Hui PanJoslin Diabetes Center, Harvard Medical School, Harvard University, Boston, MA, USA.
C Ronald KahnJoslin Diabetes Center, Harvard Medical School, Harvard University, Boston, MA, USA; Harvard Medical School, Harvard University, Boston, MA, USA. Electronic address: c.ronald.kahn@joslin.harvard.edu.

Funding

SPECIAL ASSAY COREP30DK036836 · NIDDK · JOSLIN DIABETES CENTER · PI ROHIT N. KULKARNI · 1986 to 2026
$50.5M
Developmental genes, miRNAs and adipose tissueR01DK082659 · NIDDK · JOSLIN DIABETES CENTER · PI C RONALD KAHN · 2009 to 2026
$10.7M
The Insulin Receptor and Its Signaling MechanismsR01DK031036 · NIDDK · JOSLIN DIABETES CENTER · PI C RONALD KAHN · 1986 to 2026
$4.2M
Alterations in Post-Receptor Insulin Signaling in Diabetes and Insulin ResistanceR01DK128429 · NIDDK · JOSLIN DIABETES CENTER · PI KAHN, C RONALD · 2021 to 2025
$2.9M
NIDDK NIH HHS P30 DK036836NIDDK NIH HHS R01 DK031036NIDDK NIH HHS R01 DK082659NIDDK NIH HHS R01 DK128429
6 · The paper itself

Abstract

Tissues release microRNAs (miRNAs) in small extracellular vesicles (sEVs) including exosomes, which can regulate gene expression in distal cells, thus acting as modulators of local and systemic metabolism. Here, we show that insulin regulates miRNA secretion into sEVs from 3T3-L1 adipocytes and that this process is differentially regulated from cellular expression. Thus, of the 53 miRNAs upregulated and 66 miRNAs downregulated by insulin in 3T3-L1 sEVs, only 12 were regulated in parallel in cells. Insulin regulated this process in part by phosphorylating hnRNPA1, causing it to bind to AU-rich motifs in miRNAs, mediating their secretion into sEVs. Importantly, 43% of insulin-regulated sEV-miRNAs are implicated in obesity and insulin resistance. These include let-7 and miR-103, which we show regulate insulin signaling in AML12 hepatocytes. Together, these findings demonstrate an important layer to insulin's regulation of adipose biology and provide a mechanism of tissue crosstalk in obesity and other hyperinsulinemic states.

Indexed as

Extracellular VesiclesInsulinMicroRNAs3T3-L1 CellsAdipocytesAnimalsGene Expression RegulationHepatocytesHeterogeneous Nuclear Ribonucleoprotein A1HumansInsulin ResistanceMiceObesityPhosphorylationSignal TransductionHeterogeneous Nuclear Ribonucleoprotein A1InsulinMicroRNAsadipocyteCP: MetabolismCP: Molecular biologyexosomesextracellular miRNAsinsulininsulin signalingmiRNARNA binding proteinssmall extracellular vesicles

Identifiers

PMID39002127
PMCPMC11363058

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.