Evidence map›Paper›PMID 39001800›Full record

ArticleBioinformatics (Oxford, England)2024

Automated, image-based quantification of peroxisome characteristics with perox-per-cell.

Maxwell L Neal, Nandini Shukla, Fred D Mast, Jean-Claude Farré, Therese M Pacio, Katelyn E Raney-Plourde, Sumedh Prasad, Suresh Subramani, John D Aitchison

Abstract read
In one paragraph

Article in Bioinformatics (Oxford, England), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Maxwell L NealSeattle Children's Research Institute, Center for Global Infectious Disease Research, Seattle, WA USA.
Nandini ShuklaDepartment of Molecular Biology, School of Biological Sciences, University of California, San Diego, La Jolla, CA USA.
Fred D MastSeattle Children's Research Institute, Center for Global Infectious Disease Research, Seattle, WA USA.
Jean-Claude FarréDepartment of Molecular Biology, School of Biological Sciences, University of California, San Diego, La Jolla, CA USA.
Therese M PacioSeattle Children's Research Institute, Center for Global Infectious Disease Research, Seattle, WA USA.
Katelyn E Raney-PlourdeDepartment of Molecular Biology, School of Biological Sciences, University of California, San Diego, La Jolla, CA USA.
Sumedh PrasadDepartment of Molecular Biology, School of Biological Sciences, University of California, San Diego, La Jolla, CA USA.
Suresh SubramaniDepartment of Molecular Biology, School of Biological Sciences, University of California, San Diego, La Jolla, CA USA.
John D AitchisonSeattle Children's Research Institute, Center for Global Infectious Disease Research, Seattle, WA USA.

Funding

TR&D Project 4. The Imaging Stage: Multiscale Spatiotemporal Modeling of Macromolecular Systems in Cellular NeighborhoodsP41GM109824 · NIGMS · ROCKEFELLER UNIVERSITY · PI ROUT, MICHAEL P · 2014 to 2023
$18.8M
TARGETING OF PROTEINS INTO PEROXISOMESR01DK041737 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI AITCHISON, JOHN D., SUBRAMANI, SURESH · 1990 to 2025
$8.4M
NIDDK NIH HHS R01 DK041737NIGMS NIH HHS P41 GM109824
6 · The paper itself

Abstract

summaryperox-per-cell automates cumbersome, image-based data collection tasks often encountered in peroxisome research. The software processes microscopy images to quantify peroxisome features in yeast cells. It uses off-the-shelf image processing tools to automatically segment cells and peroxisomes and then outputs quantitative metrics including peroxisome counts per cell and spatial areas. In validation tests, we found that perox-per-cell output agrees well with manually quantified peroxisomal counts and cell instances, thereby enabling high-throughput quantification of peroxisomal characteristics. AVAILABILITY AND IMPLEMENTATION: The software is coded in Python. Compiled executables and source code are available at https://github.com/AitchisonLab/perox-per-cell. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

Identifiers

PMID39001800
PMCPMC11269463

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.