Evidence map›Paper›PMID 39000535›Full record

ArticleInternational journal of molecular sciences2024

Investigation of Phosphatidylcholine by MALDI Imaging Mass Spectrometry in Normal and IVF Early-Stage Embryos.

Stefánia Gitta, Éva Szabó, Alexandra Sulc, Péter Czétány, Gábor Máté, András Balló, Tímea Csabai, Árpád Szántó, László Márk

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Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Stefánia GittaDepartment of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, Medical School, University of Pécs, 7624 Pécs, Hungary.
Éva SzabóDepartment of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, Medical School, University of Pécs, 7624 Pécs, Hungary.ORCID 0000-0002-3563-2492
Alexandra SulcDepartment of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, Medical School, University of Pécs, 7624 Pécs, Hungary.
Péter CzétányNational Laboratory on Human Reproduction, University of Pécs, 7621 Pécs, Hungary.
Gábor MátéNational Laboratory on Human Reproduction, University of Pécs, 7621 Pécs, Hungary.
András BallóNational Laboratory on Human Reproduction, University of Pécs, 7621 Pécs, Hungary.
Tímea CsabaiInstitute of Biology, Medical School, University of Pécs, 7624 Pécs, Hungary.
Árpád SzántóNational Laboratory on Human Reproduction, University of Pécs, 7621 Pécs, Hungary.
László MárkDepartment of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, Medical School, University of Pécs, 7624 Pécs, Hungary.

Funding

National Laboratory on Human Reproduction RRF-2.3.1-21-2022-00012
6 · The paper itself

Abstract

The receptive phase of the uterus is marked by structural and functional maturation of the endometrium. During this limited time span, the blastocyst competency is superimposed on the receptive endometrium. It is a well-known fact that lipid signalling in early-stage pregnancy has a crucial role in successful embryogenesis. In our study, CD-1 mouse uteri after normal and in vitro fertilization (IVF) were investigated at 6.5, 8.5, and 10.5 days of pregnancy. Matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry and liquid chromatography coupled tandem mass spectrometry were used for identification of phosphatidylcholine (PC) lipid structures. In the embryonal tissues, PC 32:0 and PC 34:0 were increased, while in the antemesometrial (AM) decidua the two 20:4-containing PCs, PC 36:4 and PC 38:4 were increased. In transferred uterus samples, higher expressions of PC 34:0, PC 34:1, PC 34:2, PC 36:1, and PC 36:2 in mesometrial decidua were seen, whereas the two 20:4-containing PCs, PC 36:4 and PC 38:4 showed increased expression in the AM and lateral decidua. This paper shows a significant spatio-temporal change in lipid metabolism during IVF procedures for the first time.

Indexed as

Fertilization in VitroPhosphatidylcholinesSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationAnimalsBlastocystEmbryo, MammalianEmbryonic DevelopmentFemaleMicePregnancyUterusPhosphatidylcholinesembryo transferendometriumimaging mass spectrometryin vitro fertilizationMALDImousephospholipidspregnancy

Identifiers

PMID39000535
PMCPMC11242196

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.