Evidence map›Paper›PMID 39000465›Full record

ArticleInternational journal of molecular sciences2024

Intestinal Epithelial Co-Culture Sensitivity to Pro-Inflammatory Stimuli and Polyphenols Is Medium-Independent.

Michelle J Haddad, Juanita Zuluaga-Arango, Hugo Mathieu, Nicolas Barbezier, Pauline M Anton

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Michelle J HaddadTransformations et Agroressources, ULR 7519, Institut Polytechnique UniLaSalle, Université d'Artois, 60000 Beauvais, France.
Juanita Zuluaga-ArangoTransformations et Agroressources, ULR 7519, Institut Polytechnique UniLaSalle, Université d'Artois, 60000 Beauvais, France.
Hugo MathieuTransformations et Agroressources, ULR 7519, Institut Polytechnique UniLaSalle, Université d'Artois, 60000 Beauvais, France.ORCID 0009-0002-9708-2796
Nicolas BarbezierTransformations et Agroressources, ULR 7519, Institut Polytechnique UniLaSalle, Université d'Artois, 60000 Beauvais, France.ORCID 0000-0003-2940-9881
Pauline M AntonTransformations et Agroressources, ULR 7519, Institut Polytechnique UniLaSalle, Université d'Artois, 60000 Beauvais, France.ORCID 0000-0002-7768-4063

Funding

Association Nationale de la Recherche et de la Technologie 2021/0204
6 · The paper itself

Abstract

The complexification of in vitro models requires the compatibility of cells with the same medium. Since immune cells are the most sensitive to growth conditions, growing intestinal epithelial cells in their usual medium seems to be necessary. This work was aimed at comparing the sensitivity of these epithelial cells to pro-inflammatory stimuli but also to dietary polyphenols in both DMEM and RPMI-1640 media. Co-cultures of Caco-2 and HT29-MTX cells were grown for 21 days in the two media before their stimulation with a cocktail of TNF-α (20 ng/mL), IL-1β (1 ng/mL), and IFN-γ (10 ng/mL) or with LPS (10 ng/mL) from

Indexed as

Coculture TechniquesIntestinal MucosaLipopolysaccharidesPolyphenolsCaco-2 CellsCatechinCulture MediaCytokinesEpithelial CellsHT29 CellsHumansInflammationTumor Necrosis Factor-alphaCatechinCulture MediaCytokinesLipopolysaccharidesPolyphenolsTumor Necrosis Factor-alphacatechinscytokine stimulationinflammatory and apoptotic pathwaysintestinal epitheliumin vitro co-culture systemsLPSRPMI-1640

Identifiers

PMID39000465
PMCPMC11242137

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.