Evidence map›Paper›PMID 39000215›Full record

ArticleInternational journal of molecular sciences2024

Long-Term In Vitro Culture Alters Gene Expression Pattern of Genes Involved in Ontological Groups Representing Cellular Processes.

Wiktoria Zgórecka, Wiesława Kranc, Małgorzata Blatkiewicz, Kacper Kamiński, Maryam Farzaneh, Artur Bryja, Paul Mozdziak, Paweł Antosik, Maciej Zabel, Marzenna Podhorska-Okołów and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Wiktoria ZgóreckaDepartment of Anatomy, Poznan University of Medical Sciences, 60-781 Poznan, Poland.ORCID 0009-0006-0341-9781
Wiesława KrancDepartment of Anatomy, Poznan University of Medical Sciences, 60-781 Poznan, Poland.ORCID 0000-0001-7518-4834
Małgorzata BlatkiewiczDepartment of Histology and Embryology, Poznan University of Medical Sciences, 60-812 Poznan, Poland.ORCID 0000-0001-5506-2397
Kacper KamińskiDepartment of Histology and Embryology, Poznan University of Medical Sciences, 60-812 Poznan, Poland.ORCID 0000-0003-0641-1444
Maryam FarzanehFertility, Infertility and Perinatology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.ORCID 0000-0001-6239-8745
Artur BryjaDivision of Anatomy, Department of Human Morphology and Embryology, Faculty of Medicine, Wroclaw Medical University, 50-368 Wroclaw, Poland.
Paul MozdziakPhysiology Graduate Program, North Carolina State University, Raleigh, NC 27695, USA.ORCID 0000-0002-1575-3123
Paweł AntosikDepartment of Veterinary Surgery, Nicolaus Copernicus University in Toruń, 87-100 Toruń, Poland.
Maciej ZabelDivision of Anatomy and Histology, University of Zielona Góra, 65-417 Zielona Góra, Poland.
Marzenna Podhorska-OkołówDivision of Ultrastructural Research, Faculty of Medicine, Wroclaw Medical University, 50-368 Wroclaw, Poland.
Piotr DzięgielDivision of Histology and Embryology, Department of Human Morphology and Embryology Faculty of Medicine, Wroclaw Medical University, 50-368 Wroclaw, Poland.
Bartosz KempistyDivision of Anatomy, Department of Human Morphology and Embryology, Faculty of Medicine, Wroclaw Medical University, 50-368 Wroclaw, Poland.
Dorota BukowskaDepartment of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in Toruń, 87-100 Toruń, Poland.

Funding

National Institute of Food and Agriculture Hatch project nc07082
6 · The paper itself

Abstract

The oviduct provides an optimal environment for the final preparation, transport, and survival of gametes, the fertilization process, and early embryonic development. Most of the studies on reproduction are based on in vitro cell culture models because of the cell's accessibility. It creates opportunities to explore the complexity of directly linked processes between cells. Previous studies showed a significant expression of genes responsible for cell differentiation, maturation, and development during long-term porcine oviduct epithelial cells (POECs) in vitro culture. This study aimed at establishing the transcriptomic profile and comprehensive characteristics of porcine oviduct epithelial cell in vitro cultures, to compare changes in gene expression over time and deliver information about the expression pattern of genes highlighted in specific GO groups. The oviduct cells were collected after 7, 15, and 30 days of in vitro cultivation. The transcriptomic profile of gene expression was compared to the control group (cells collected after the first day). The expression of

Indexed as

Epithelial CellsTranscriptomeAnimalsCell Culture TechniquesCells, CulturedFallopian TubesFemaleGene Expression ProfilingGene Expression RegulationOviductsSwinecell adhesioncell migrationintercellular communicationreproductive biologyRNA processing

Identifiers

PMID39000215
PMCPMC11241590

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.