Evidence map›Paper›PMID 39000202›Full record

ArticleInternational journal of molecular sciences2024

Ultrasound-Mediated Lysozyme Microbubbles Targeting NOX4 Knockdown Alleviate Cisplatin-Exposed Cochlear Hair Cell Ototoxicity.

Yuan-Yung Lin, Ai-Ho Liao, Hsiang-Tzu Li, Peng-Yi Jiang, Yi-Chun Lin, Ho-Chiao Chuang, Kuo-Hsing Ma, Hang-Kang Chen, Yi-Tsen Liu, Cheng-Ping Shih and 1 more

Abstract read
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In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yuan-Yung LinGraduate Institute of Medical Sciences, National Defense Medical Center, No. 161, Sec. 6, Minquan E. Road, Taipei 114201, Taiwan.ORCID 0000-0001-9571-8960
Ai-Ho LiaoGraduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 106335, Taiwan.ORCID 0000-0001-9101-6662
Hsiang-Tzu LiGraduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 106335, Taiwan.
Peng-Yi JiangGraduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 106335, Taiwan.
Yi-Chun LinDepartment of Otolaryngology-Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical Center, No. 325, Section 2, Cheng-Kung Road, Taipei 11490, Taiwan.ORCID 0000-0002-7984-2395
Ho-Chiao ChuangDepartment of Mechanical Engineering, National Taipei University of Technology, Taipei 106344, Taiwan.
Kuo-Hsing MaDepartment of Biology and Anatomy, National Defense Medical Center, Taipei 114201, Taiwan.
Hang-Kang ChenGraduate Institute of Medical Sciences, National Defense Medical Center, No. 161, Sec. 6, Minquan E. Road, Taipei 114201, Taiwan.ORCID 0000-0002-5344-2019
Yi-Tsen LiuGraduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 106335, Taiwan.
Cheng-Ping ShihDepartment of Otolaryngology-Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical Center, No. 325, Section 2, Cheng-Kung Road, Taipei 11490, Taiwan.ORCID 0000-0002-5515-2509
Chih-Hung WangGraduate Institute of Medical Sciences, National Defense Medical Center, No. 161, Sec. 6, Minquan E. Road, Taipei 114201, Taiwan.ORCID 0000-0001-5058-4356

Funding

Medical Affairs Bureau, Ministry of National Defense, Taiwan MAB-D-111145 to C.-H.W.National Science and Technology Council, Taiwan NSTC112-2221-E-011-026-MY3 and NSTC108-2221-E-011-161-MY4 to A.-H.L., NSTC111-2314-B-016-040, and NSTC109-2314-B-016-005 to C.-P.S., and NSTC110-2314-B-075-086-MY3 to C.-H.W.National Taiwan University of Science and Technology/Tri-Service General Hospital Joint Research Program NTUST-TSGH-112-01 and NTUST-TSGH-113-01 to A.-H.L.Teh-Tzer Study Group for Human Medical Research Foundation A1101021 to C.-H.W.Tri-Service General Hospital TSGH-D-112059, TSGH-D-109058 and TSGH-C108-040 to Y.-Y.L., TSGH-A-112002 to C.-P.S. and TSGH-D-112059, TSGH-D-111059 to C.-H.W.
6 · The paper itself

Abstract

The nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 4 (NOX4) protein plays an essential role in the cisplatin (CDDP)-induced generation of reactive oxygen species (ROS). In this study, we evaluated the suitability of ultrasound-mediated lysozyme microbubble (USMB) cavitation to enhance NOX4 siRNA transfection in vitro and ex vivo. Lysozyme-shelled microbubbles (LyzMBs) were constructed and designed for siNOX4 loading as siNOX4/LyzMBs. We investigated different siNOX4-based cell transfection approaches, including naked siNOX4, LyzMB-mixed siNOX4, and siNOX4-loaded LyzMBs, and compared their silencing effects in CDDP-treated HEI-OC1 cells and mouse organ of Corti explants. Transfection efficiencies were evaluated by quantifying the cellular uptake of cyanine 3 (Cy3) fluorescein-labeled siRNA. In vitro experiments showed that the high transfection efficacy (48.18%) of siNOX4 to HEI-OC1 cells mediated by US and siNOX4-loaded LyzMBs significantly inhibited CDDP-induced ROS generation to almost the basal level. The ex vivo CDDP-treated organ of Corti explants of mice showed an even more robust silencing effect of the NOX4 gene in the siNOX4/LyzMB groups treated with US sonication than without US sonication, with a marked abolition of CDDP-induced ROS generation and cytotoxicity. Loading of siNOX4 on LyzMBs can stabilize siNOX4 and prevent its degradation, thereby enhancing the transfection and silencing effects when combined with US sonication. This USMB-derived therapy modality for alleviating CDDP-induced ototoxicity may be suitable for future clinical applications.

Indexed as

CisplatinHair Cells, AuditoryMicrobubblesMuramidaseNADPH Oxidase 4OtotoxicityReactive Oxygen SpeciesAnimalsCell LineGene Knockdown TechniquesMiceRNA, Small InterferingUltrasonic WavesCisplatinMuramidaseNADPH Oxidase 4Nox4 protein, mouseReactive Oxygen SpeciesRNA, Small Interferingcavitationcisplatin (CDDP)cochlear hair cellgene knockdownmicrobubblesNADPH oxidase-4 (NOX4)reactive oxygen species (ROS)small interfering RNA (siRNA)ultrasound

Identifiers

PMID39000202
PMCPMC11241201

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.