Evidence map›Paper›PMID 39000162›Full record

ArticleInternational journal of molecular sciences2024

Reversed Corneal Fibroblasts Therapy Restores Transparency of Mouse Cornea after Injury.

Maria A Surovtseva, Kristina Yu Krasner, Irina I Kim, Nikolay V Surovtsev, Elena V Chepeleva, Natalia A Bondarenko, Alexander P Lykov, Nataliya P Bgatova, Alina A Alshevskaya, Alexander N Trunov and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Maria A SurovtsevaResearch Institute of Clinical and Experimental Lymphology Branch of Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 2 Timakova Str., 630060 Novosibirsk, Russia.
Kristina Yu KrasnerResearch Institute of Clinical and Experimental Lymphology Branch of Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 2 Timakova Str., 630060 Novosibirsk, Russia.ORCID 0000-0002-1921-8072
Irina I KimResearch Institute of Clinical and Experimental Lymphology Branch of Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 2 Timakova Str., 630060 Novosibirsk, Russia.ORCID 0000-0002-7380-2763
Nikolay V SurovtsevInstitute of Automation and Electrometry, Russian Academy of Sciences, 1 Academician Koptyug St., 630090 Novosibirsk, Russia.ORCID 0000-0002-1580-0508
Elena V ChepelevaResearch Institute of Clinical and Experimental Lymphology Branch of Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 2 Timakova Str., 630060 Novosibirsk, Russia.ORCID 0000-0003-0715-4451
Natalia A BondarenkoResearch Institute of Clinical and Experimental Lymphology Branch of Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 2 Timakova Str., 630060 Novosibirsk, Russia.ORCID 0000-0002-8443-656X
Alexander P LykovResearch Institute of Clinical and Experimental Lymphology Branch of Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 2 Timakova Str., 630060 Novosibirsk, Russia.ORCID 0000-0003-4897-8676
Nataliya P BgatovaResearch Institute of Clinical and Experimental Lymphology Branch of Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 2 Timakova Str., 630060 Novosibirsk, Russia.ORCID 0000-0002-4507-093X
Alina A AlshevskayaFederal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Russian Federation, 2, Building 4 Bolshaya Pirogovskaya St., 119048 Moscow, Russia.
Alexander N TrunovNovosibirsk Branch of S. Fedorov Eye Microsurgery Federal State Institution, 10 Kolkhidskaya Str., 630096 Novosibirsk, Russia.ORCID 0000-0002-7592-8984
Valery V ChernykhNovosibirsk Branch of S. Fedorov Eye Microsurgery Federal State Institution, 10 Kolkhidskaya Str., 630096 Novosibirsk, Russia.
Olga V PoveshchenkoResearch Institute of Clinical and Experimental Lymphology Branch of Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 2 Timakova Str., 630060 Novosibirsk, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell-based therapies using corneal stromal stem cells (CSSC), corneal keratocytes, or a combination of both suppress corneal scarring. The number of quiescent keratocytes in the cornea is small; it is difficult to expand them in vitro in quantities suitable for transplantation. This study examined the therapeutic effect of corneal fibroblasts reversed into keratocytes (rCF) in a mouse model of mechanical corneal injury. The therapeutic effect of rCF was studied in vivo (slit lamp, optical coherence tomography) and ex vivo (transmission electron microscopy and immunofluorescence staining). Injection of rCF into the injured cornea was accompanied by recovery of corneal thickness, improvement of corneal transparency, reduction of type III collagen in the stroma, absence of myofibroblasts, and the improvement in the structural organization of collagen fibers. TEM results showed that 2 months after intrastromal injection of cells, there was a decrease in the fibril density and an increase in the fibril diameter and the average distance between collagen fibrils. The fibrils were well ordered and maintained the short-range order and the number of nearest-neighbor fibrils, although the averaged distance between them increased. Our results demonstrated that the cell therapy of rCF from ReLEx SMILe lenticules promotes the recovery of transparent corneal stroma after injury.

Indexed as

Corneal InjuriesFibroblastsAnimalsCell- and Tissue-Based TherapyCorneaCorneal KeratocytesCorneal StromaDisease Models, AnimalMiceTomography, Optical Coherencecell therapycorneal stromal scarkeratocytesmouse corneal injuryreversion

Identifiers

PMID39000162
PMCPMC11241278

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.