Evidence map›Paper›PMID 38998471›Full record

ArticleFoods (Basel, Switzerland)2024

Isolation, Characterization and IgE Binding of Two 2S Albumins of Pomegranate Seeds.

Lisa Tuppo, Claudia Alessandri, Laura Zaccaro, Ivana Giangrieco, Maurizio Tamburrini, Adriano Mari, Maria Antonietta Ciardiello

Abstract read
In one paragraph

Article in Foods (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Lisa TuppoInstitute of Biosciences and BioResources (IBBR), National Research Council of Italy (CNR), 80131 Naples, Italy.ORCID 0009-0001-5673-6629
Claudia AlessandriAssociated Centers for Molecular Allergology (CAAM), 00100 Rome, Italy.
Laura ZaccaroInstitute of Biostructures and Bioimaging (IBB), National Research Council of Italy (CNR), 80131 Naples, Italy.ORCID 0000-0001-6843-0152
Ivana GiangriecoInstitute of Biosciences and BioResources (IBBR), National Research Council of Italy (CNR), 80131 Naples, Italy.ORCID 0000-0002-1153-4249
Maurizio TamburriniInstitute of Biosciences and BioResources (IBBR), National Research Council of Italy (CNR), 80131 Naples, Italy.
Adriano MariAssociated Centers for Molecular Allergology (CAAM), 00100 Rome, Italy.
Maria Antonietta CiardielloInstitute of Biosciences and BioResources (IBBR), National Research Council of Italy (CNR), 80131 Naples, Italy.ORCID 0000-0002-7203-0554

Funding

project PNRR, Modelli per un'alimentazione sostenibile, "ON Foods"-Research and Innovation Network on Food and Nutrition Sustainability, Safety and Se-curity-Working ON Foods PE00000003, PE10
6 · The paper itself

Abstract

Literature reports suggest that the presence of proteins in pomegranate seeds is responsible for sensitization and IgE-mediated allergic reactions. The objective of this study was the analysis of a pomegranate seed extract and the isolation and characterization of proteins contained in high amounts. The extract characterization showed a protein profile with main bands at about 18 kDa and below 10 kDa upon SDS-PAGE, and molecules were recognized by specific IgEs upon immunoblotting. Then, two new 2S albumins, a monomeric and a heterodimeric one, were isolated by using classical biochemical methods. They were identified via direct protein sequencing and mass spectrometry, and their primary structure was analyzed and compared with homologous allergenic proteins via bioinformatics. In an Italian population of 703 suspected allergic patients, analyzed by using the FABER

Indexed as

bioinformaticsfood allergyimmunoblottingItalian populationmass spectrometryN-terminal amino acid sequencingprotein biochemistryprotein purificationseed storage proteinssensitization

Identifiers

PMID38998471
PMCPMC11241328

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.