Light-Sheet Microscopy Enables Three-Dimensional Fluorescence Imaging and Live Imaging of Satellite Cells on Skeletal Muscle Fibers.

Hira Asif Khan, Nick Van Hateren, Anne-Gaëlle Borycki

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Hira Asif KhanSchool of Biosciences, University of Sheffield, Firth Court, Western Bank, Sheffield, UK.

Nick Van HaterenThe Wolfson Light Microscopy Facility, University of Sheffield, Firth Court, Western Bank, Sheffield, UK.

Anne-Gaëlle BoryckiSchool of Biosciences, University of Sheffield, Firth Court, Western Bank, Sheffield, UK. a.g.borycki@sheffield.ac.uk.

Funding

BBSRC ALERT14 BB/M012522/1

6 · The paper itself

Abstract

The ex vivo myofiber culture system has proven to be a useful methodology to explore the biology and behavior of satellite cells within their niche environment. However, a limitation of this system is that myofibers and their associated satellite cells are commonly examined using conventional fluorescence microscopy, which renders a three-dimensional system into two-dimensional imaging, leading to the loss of precious information or misleading interpretation of observations. Here, we report on the use of light-sheet fluorescence microscopy to generate three-dimensional and live imaging of satellite cells on myofibers. Light-sheet microscopy offers high imaging speed and good spatial resolution with minimal photo-bleaching, allowing live imaging and three-dimensional acquisition of skeletal muscle fiber specimen. The potentials of this technology are wide, ranging from the visualization of satellite cell behavior such as cell division and cell migration to imaging the sub-cellular localization of proteins or organelles.

Indexed as

Imaging, Three-DimensionalMuscle Fibers, SkeletalSatellite Cells, Skeletal MuscleAnimalsCells, CulturedMiceMicroscopy, FluorescenceImmunofluorescenceLight-sheet microscopyLive imagingSatellite cellSkeletal muscle fiber

Identifiers

PMID38997538

DOI10.1007/978-1-4939-3810-0_11

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.