Evidence map›Paper›PMID 38997516›Full record

ArticleInternational journal of legal medicine2024

Statistical methods for discrimination of STR genotypes using high resolution melt curve data.

Darianne C Cloudy, Edward L Boone, Kristi Kuehnert, Chastyn Smith, Jordan O Cox, Sarah J Seashols-Williams, Tracey Dawson Green

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of legal medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Darianne C CloudyDepartment of Forensic Science, Virginia Commonwealth University, 1015 Floyd Avenue, PO Box 843079, Richmond, VA , 23284, USA.ORCID http://orcid.org/0009-0008-1125-6622
Edward L BooneDepartment of Statistical Sciences and Operations Research, Virginia Commonwealth University, 1015 Floyd Avenue, PO Box 843079, Richmond, VA, 23284, USA.ORCID http://orcid.org/0000-0003-0755-6899
Kristi KuehnertDepartment of Forensic Science, Virginia Commonwealth University, 1015 Floyd Avenue, PO Box 843079, Richmond, VA , 23284, USA.ORCID http://orcid.org/0009-0008-5669-9654
Chastyn SmithDepartment of Forensic Science, Virginia Commonwealth University, 1015 Floyd Avenue, PO Box 843079, Richmond, VA , 23284, USA. smithc13@vcu.edu.ORCID http://orcid.org/0000-0003-1353-6034
Jordan O CoxDepartment of Forensic Science, Virginia Commonwealth University, 1015 Floyd Avenue, PO Box 843079, Richmond, VA , 23284, USA.ORCID http://orcid.org/0000-0003-4483-9185
Sarah J Seashols-WilliamsDepartment of Forensic Science, Virginia Commonwealth University, 1015 Floyd Avenue, PO Box 843079, Richmond, VA , 23284, USA.ORCID http://orcid.org/0000-0003-0093-8261
Tracey Dawson GreenDepartment of Forensic Science, Virginia Commonwealth University, 1015 Floyd Avenue, PO Box 843079, Richmond, VA , 23284, USA.ORCID http://orcid.org/0000-0003-4761-6657

Funding

National Institute of Justice 2015-MU-MU-K026
6 · The paper itself

Abstract

Despite the improvements in forensic DNA quantification methods that allow for the early detection of low template/challenged DNA samples, complicating stochastic effects are not revealed until the final stage of the DNA analysis workflow. An assay that would provide genotyping information at the earlier stage of quantification would allow examiners to make critical adjustments prior to STR amplification allowing for potentially exclusionary information to be immediately reported. Specifically, qPCR instruments often have dissociation curve and/or high-resolution melt curve (HRM) capabilities; this, coupled with statistical prediction analysis, could provide additional information regarding STR genotypes present. Thus, this study aimed to evaluate Qiagen's principal component analysis (PCA)-based ScreenClust

Indexed as

DNA FingerprintingGenotypeMicrosatellite RepeatsPrincipal Component AnalysisReal-Time Polymerase Chain ReactionDiscriminant AnalysisHumansSoftwareHigh resolution melt curvesLinear Discriminant Analysis (LDA)Prediction modelingPrincipal component analysis (PCA)qPCR genotyping

Identifiers

PMID38997516
PMCPMC11490427

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.