Evidence map›Paper›PMID 38995923›Full record

ArticlePloS one2024

The differentiation of Lgr5+ progenitor cells on nanostructures of self-assembled silica beads.

Wenjun Cai, Zhichun Huang, Baobin Sun, Ling Lu, Xiaoqiong Ding, Feng Tao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Wenjun CaiDepartment of Otorhinolaryngology-Head and Neck Surgery, Zhong Da Hospital, Southeast University, Nanjing, China.ORCID 0000-0002-8205-3346
Zhichun HuangDepartment of Otorhinolaryngology-Head and Neck Surgery, Zhong Da Hospital, Southeast University, Nanjing, China.
Baobin SunDepartment of Otorhinolaryngology-Head and Neck Surgery, Zhong Da Hospital, Southeast University, Nanjing, China.
Ling LuDepartment of Otorhinolaryngology-Head and Neck Surgery, Zhong Da Hospital, Southeast University, Nanjing, China.
Xiaoqiong DingDepartment of Otorhinolaryngology-Head and Neck Surgery, Zhong Da Hospital, Southeast University, Nanjing, China.
Feng TaoDepartment of Otorhinolaryngology-Head and Neck Surgery, Zhong Da Hospital, Southeast University, Nanjing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Supporting cells(SCs) have been demonstrated to be a reliable source for regenerating hair cells(HCs). Previous research has reported that Lgr5+ SCs can regenerate HCs both in vitro and in vivo. However, there is limited knowledge about the impact of the material on Lgr5+ cells. In this study, Lgr5+ cells were isolated from neonatal Lgr5-EGFP-CreERT2 transgenic mice by flow cytometry and then plated on self-assembled silica beads (SB). Lgr5+ cell differentiation was observed by immunofluorescence. We found that in the direct differentiation assay, the SB group generated more hair cells than the control group(*p < 0.05). Especially in the SB group, Lgr5+ progenitors generated significantly more Myo7a+ HCs outside of the colony than in the control group(**p < 0.01). In the sphere differentiation assay, we found that the diameter of spheres in the SB group was significantly larger compared to those of the control group(**p < 0.01). However, the difference in the ratio of myo7a+ cell counts was not obvious(P>0.05). The experiment proved that the self-assembled silica beads could promote the differentiation of Lgr5+ progenitors in vitro. Our findings implicate that nanostructures of self-assembled silica beads can be used as vectors for stem cell research in the inner ear.

Indexed as

Cell DifferentiationNanostructuresReceptors, G-Protein-CoupledSilicon DioxideStem CellsAnimalsCells, CulturedMiceMice, TransgenicLgr5 protein, mouseReceptors, G-Protein-CoupledSilicon Dioxide

Identifiers

PMID38995923
PMCPMC11244819

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.