Integrating HexNAcQuest with Glycoproteomics Data Analysis Software to Distinguish HexNAc Isomers on Peptides.

Chunyan Hou, Weiyu Li, Yaoxiang Li, Junfeng Ma

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Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 authors.

Chunyan HouDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, USA.

Weiyu LiDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, USA.

Yaoxiang LiDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, USA.

Junfeng MaDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, USA. junfeng.ma@georgetown.edu.ORCID 0000-0002-5183-5425

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6 · The paper itself

Abstract

Recently, HexNAcQuest was developed to help distinguish peptides modified by HexNAc isomers, more specifically O-linked β-N-acetylglucosamine (O-GlcNAc) and O-linked α-N-acetylgalactosamine (O-GalNAc, Tn antigen). To facilitate its usage (particularly for datasets from glycoproteomics studies), herein we present a detailed protocol. It describes example cases and procedures for which users might need to use HexNAcQuest to distinguish these two modifications.

Indexed as

GlycomicsPeptidesSoftwareAcetylgalactosamineAcetylglucosamineIsomerismProteomicsAcetylgalactosamineAcetylglucosaminePeptidesGlycoproteomicsHexNAcHexNAcQuestO-GlcNAcTn antigen

Identifiers

PMID38995536

DOI10.1038/nmeth.4256

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