Evidence map›Paper›PMID 38993099›Full record

ArticleInternational journal of stem cells2024

Establishing Three-Dimensional Explant Culture of Human Dental Pulp Tissue.

Eun Jin Seo, Soyoung Park, Eungyung Lee, Yang Hoon Huh, Ye Eun Ha, Gabor J Tigyi, Taesung Jeong, Il Ho Jang, Jonghyun Shin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of stem cells, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Eun Jin SeoDental and Life Science Institute, School of Dentisty, Pusan National University, Yangsan, Korea.ORCID https://orcid.org/0000-0002-9416-9020
Soyoung ParkDental and Life Science Institute, School of Dentisty, Pusan National University, Yangsan, Korea.ORCID https://orcid.org/0000-0003-3264-4014
Eungyung LeeDental and Life Science Institute, School of Dentisty, Pusan National University, Yangsan, Korea.ORCID https://orcid.org/0000-0001-8313-8571
Yang Hoon HuhCenter for Electron Microscopy Research, Korea Basic Science Institute, Ochang, Korea.ORCID https://orcid.org/0000-0003-3157-304X
Ye Eun HaTissue Regeneration Laboratory, StemDen Co., Ltd., Yangsan, Korea.ORCID https://orcid.org/0009-0008-2523-7975
Gabor J TigyiDepartment of Physiology, The University of Tennessee Health Science Center, Memphis, TN, USA.ORCID https://orcid.org/0000-0001-5371-171X
Taesung JeongDental and Life Science Institute, School of Dentisty, Pusan National University, Yangsan, Korea.ORCID https://orcid.org/0000-0002-0431-5574
Il Ho JangDental and Life Science Institute, School of Dentisty, Pusan National University, Yangsan, Korea.ORCID https://orcid.org/0000-0002-0820-3035
Jonghyun ShinDental and Life Science Institute, School of Dentisty, Pusan National University, Yangsan, Korea.ORCID https://orcid.org/0000-0002-9777-0196

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mesenchymal stem cells in the dental tissue indicate a disposition for differentiation into diverse dental lineages and contain enormous potential as the important means for regenerative medicine in dentistry. Among various dental tissues, the dental pulp contains stem cells, progenitor cells and odontoblasts for maintaining dentin homeostasis. The conventional culture of stem cells holds a limit as the living tissue constitutes the three-dimensional (3D) structure. Recent development in the organoid cultures have successfully recapitulated 3D structure and advanced to the assembling of different types. In the current study, the protocol for 3D explant culture of the human dental pulp tissue has been established by adopting the organoid culture. After isolating dental pulp from human tooth, the intact tissue was placed between two layers for Matrigel with addition of the culture medium. The reticular outgrowth of pre-odontoblast layer continued for a month and the random accumulation of dentin was observed near the end. Electron microscopy showed the cellular organization and

Indexed as

Cell culture techniquesDental pulp stem cellsExplant cultureOdontoblaststhree dimensional

Identifiers

PMID38993099
PMCPMC11361853

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.