ReviewMolecular diagnosis & therapy2024
Clinical Metagenomic Next-Generation Sequencing for Diagnosis of Central Nervous System Infections: Advances and Challenges.
Review in Molecular diagnosis & therapy, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed.
- Etiologic diagnosis of suspected tuberculous meningitis by multiplex PCR of cerebrospinal fluid.Journal of clinical microbiology · 2026Article
- Clinical utility of metagenomic next-generation sequencing in precision diagnosis of infectious diseases: a retrospective study based on bronchoalveolar lavage fluid, blood, and cerebrospinal fluid.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Validation of an integrated metagenomic pipeline combining optimized wet-lab processing and tiered reporting for CSF pathogen detection.Microbiology spectrum · 2026Article
- Review
- Metagenomic next-generation sequencing assists in identifying neurosyphilis: a case series.Infection · 2026Article
- A successful individualized comprehensive therapy for carbapenem-resistant Klebsiella pneumoniae induced multifocal brain abscesses: a case report and literature review.BMC infectious diseases · 2026Review
- Clinical and laboratory profiles of acute central nervous system infections in a Chinese pediatric cohort: a five-year retrospective analysis.BMC pediatrics · 2026Article
- [Advances in the Application of Artificial Intelligence in Clinical Microbiological Testing].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2026Review
- Integrating metagenomics and metatranscriptomics intoThe Journal of general virology · 2026Review
- Evaluation of the diagnostic value of metagenomic next-generation sequencing for zoonotic cestodeFood and waterborne parasitology · 2026Article
- C-arm-guided percutaneous biopsy combined with mNGS: a dual-modality strategy for optimizing diagnosis and targeted management of spinal infections.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2026Review
- The value of mNGS in the diagnosis of central nervous system infections in immunodeficient hosts with decompensated cirrhosis complicated byFrontiers in medicine · 2026Article
- Targeted next-generation sequencing for pediatric community-acquired pneumonia pathogen detection: a single-center study.Frontiers in cellular and infection microbiology · 2026Article
- Fatal Pharyngeal Cellulitis Caused byInfection and drug resistance · 2026Article
- Evaluating the Clinical Impact of Metagenomic Next-Generation Sequencing in CNS Infections: A Diagnostic Pathway and Resource Utilization Modeling Study.Open forum infectious diseases · 2026Article
- Metagenomic next-generation sequencing reveals cross-reactivity of lateral flow cryptococcal antigen assay withASM case reports · 2026Article
- Clinicopathologic Features of Genitourinary Malakoplakia and Analytical Utility of the MetaPath Assay.Infection and drug resistance · 2026Article
- Case Report: FromFrontiers in immunology · 2026Article
- A case of infective endocarditis caused byIDCases · 2026Article
- Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Central nervous system (CNS) infections carry a substantial burden of morbidity and mortality worldwide, and accurate and timely diagnosis is required to optimize management. Metagenomic next-generation sequencing (mNGS) has proven to be a valuable tool in detecting pathogens in patients with suspected CNS infection. By sequencing microbial nucleic acids present in a patient's cerebrospinal fluid, brain tissue, or samples collected outside of the CNS, such as plasma, mNGS can detect a wide range of pathogens, including rare, unexpected, and/or fastidious organisms. Furthermore, its target-agnostic approach allows for the identification of both known and novel pathogens. This is particularly useful in cases where conventional diagnostic methods fail to provide an answer. In addition, mNGS can detect multiple microorganisms simultaneously, which is crucial in cases of mixed infections without a clear predominant pathogen. Overall, clinical mNGS testing can help expedite the diagnostic process for CNS infections, guide appropriate management decisions, and ultimately improve clinical outcomes. However, there are key challenges surrounding its use that need to be considered to fully leverage its clinical impact. For example, only a few specialized laboratories offer clinical mNGS due to the complexity of both the laboratory methods and analysis pipelines. Clinicians interpreting mNGS results must be aware of both false negatives-as mNGS is a direct detection modality and requires a sufficient amount of microbial nucleic acid to be present in the sample tested-and false positives-as mNGS detects environmental microbes and their nucleic acids, despite best practices to minimize contamination. Additionally, current costs and turnaround times limit broader implementation of clinical mNGS. Finally, there is uncertainty regarding the best practices for clinical utilization of mNGS, and further work is needed to define the optimal patient population(s), syndrome(s), and time of testing to implement clinical mNGS.
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Registered trials
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