Evidence map›Paper›PMID 38990374›Full record

ArticleMikrochimica acta2024

Fluorescent DNA tetrahedral probe with catalytic hairpin self-assembly reaction for imaging of miR-21 and miR-155 in living cells.

Shan Wang, Jiejie Guang, Yahui Gao, Bingyuan Fan, Yan Liang, Jinru Pan, Li Li, Wei Meng, Fang Hu

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Shan Wang *Key Laboratory of Biomedical Functional Materials, School of Sciences, China Pharmaceutical University, Nanjing, 211198, China.
Jiejie Guang *Key Laboratory of Biomedical Functional Materials, School of Sciences, China Pharmaceutical University, Nanjing, 211198, China.
Yahui GaoKey Laboratory of Biomedical Functional Materials, School of Sciences, China Pharmaceutical University, Nanjing, 211198, China.
Bingyuan FanKey Laboratory of Biomedical Functional Materials, School of Sciences, China Pharmaceutical University, Nanjing, 211198, China.
Yan LiangKey Laboratory of Biomedical Functional Materials, School of Sciences, China Pharmaceutical University, Nanjing, 211198, China.
Jinru PanKey Laboratory of Biomedical Functional Materials, School of Sciences, China Pharmaceutical University, Nanjing, 211198, China.
Li LiNanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, 210008, China. njgllily@163.com.
Wei MengKey Laboratory of Biomedical Functional Materials, School of Sciences, China Pharmaceutical University, Nanjing, 211198, China. mengwei@cpu.edu.cn.
Fang HuKey Laboratory of Biomedical Functional Materials, School of Sciences, China Pharmaceutical University, Nanjing, 211198, China. hufang@cpu.edu.cn.

Funding

National Natural Science Foundation of China 82072058National Natural Science Foundation of China 82230070
6 · The paper itself

Abstract

A CHA-based fluorescent DNA tetrahedral probe (FDTp) has been designed to detect the microRNAs miR-21 and miR-155 sensitively and specifically in living cells. The design consisted of functional elements (H1, H2, and Protector) connected to a DNA tetrahedron modified with two pairs of fluorophores and quenching groups. In the presence of miR-21, the chain displacement effect was triggered and Cy3 fluorescence was emitted. In the presence of miR-155, the signal of the catalytic hairpin assembly (CHA) between H1 and H2 on FDTp was amplified, making the fluorescence of FAM sensitive to miR-155. Using this method, the detection limit for miR-155 was 5 pM. The FDTp successfully imaged miR-21 and miR-155 in living cells and distinguished a variety of cell lines based on their expression levels of miR-21 and miR-155. The detection and imaging of dual targets in this design ensured the accuracy of tumor diagnosis and provided a new method for early tumor diagnosis.

Indexed as

Fluorescent DyesMicroRNAsCatalysisDNADNA ProbesHeLa CellsHumansInverted Repeat SequencesLimit of DetectionOptical ImagingSpectrometry, FluorescenceDNADNA ProbesFluorescent DyesMicroRNAsMIRN155 microRNA, humanMIRN21 microRNA, humanCatalytic hairpin assemblyFluorescence resonance energy transferFluorescent DNA tetrahedral probeLiving cell imagingMicroRNAs

Identifiers

PMID38990374

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.