ArticleFrontiers in veterinary science2024
Adapting the SMART tube technology for flow cytometry in feline full blood samples.
Article in Frontiers in veterinary science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Coordinated expansion of CD163bioRxiv : the preprint server for biology · 2026Article
- Feline leukocyte immunophenotyping: an optimised whole-blood flow cytometry protocol.MethodsX · 2026Article
- Flow cytometric evaluation of monoclonal antibodies for cross-reactivity with feline leukocytes.Frontiers in veterinary science · 2026Article
- Article
- Cost-effective in-house-made whole blood materials for internal quality control in clinical flow cytometry analysis.Analytical and bioanalytical chemistry · 2025Article
- Whole blood RNA profiling in cats dissects the host immunological response during recovery from feline infectious peritonitis.PloS one · 2025Article
- Flow cytometric features of lymphoid subsets in healthy and diseased cats.Frontiers in veterinary science · 2025Article
- High-parameter immunophenotyping reveals distinct immune cell profiles in pruritic dogs and cats.Frontiers in veterinary science · 2024Article
- Large-cell lymphoma in four cats after successful treatment of feline infectious peritonitis with oral GS-441524: a novel clinical observation.Journal of feline medicine and surgeryArticle
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Authors and funding
9 authors.
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Abstract
Flow cytometry of blood samples is a very valuable clinical and research tool to monitor the immune response in human patients. Furthermore, it has been successfully applied in cats, such as for infections with feline immune deficiency virus (FIV). However, if cells are not isolated and frozen, analysis of anticoagulated blood samples requires mostly prompt processing following blood collection, making later analysis of stored full blood samples obtained in clinical studies often impossible. The SMART Tube system (SMART TUBE Inc., California, United States; SMT) allows fixation and long-term preservation of whole blood samples at -80°C. However, this system has so far only been applied to human biological samples. In the present study, a new flow cytometry SMART Tube protocol adapted for feline whole blood samples was successfully established allowing quantification of T-helper cells, cytotoxic T-cells, B-cells, monocytes, and neutrophils up to 2 years post sampling. Results obtained from frozen stabilized and fresh blood samples were compared for validation purposes and correlated to differential blood counts from a conventional hematology analyzer. Clinical applicability of the new technique was verified by using samples from a treatment study for feline infectious peritonitis (FIP). Using the new SMT protocol on retained samples, it could be demonstrated that long-term storage of these SMT tubes is also possible. In summary, the newly adapted SMT protocol proved suitable for performing flow cytometry analysis on stored feline whole blood samples, thus opening up new avenues for veterinary research on a variety of aspects of clinical interest.
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