Evidence map›Paper›PMID 38988319›Full record

ArticleJournal of cell science2024

Evidence of 14-3-3 proteins contributing to kinetochore integrity and chromosome congression during mitosis.

Guhan Kaliyaperumal Anbalagan, Prakhar Agarwal, Santanu Kumar Ghosh

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Guhan Kaliyaperumal AnbalaganDepartment of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Powai, 400 076, India.ORCID 0000-0001-7823-7153
Prakhar AgarwalDepartment of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Powai, 400 076, India.ORCID 0000-0001-5794-545X
Santanu Kumar GhoshDepartment of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Powai, 400 076, India.ORCID 0000-0002-3190-8084

Funding

Understanding the role of RNA-binding protein mutations in cancerP20GM113132 · NIGMS · DARTMOUTH COLLEGE · PI MIERKE, DALE F · 2016 to 2025
$25.9M
Targeting Tumors with NF1 LossR01NS095411 · NINDS · DARTMOUTH COLLEGE · PI HOOPES, JACK, RATNER, NANCY · 2015 to 2019
$2.7M
Defining human kinase-substrate networks and their dynamic regulationR01GM122846 · NIGMS · DARTMOUTH COLLEGE · PI GERBER, SCOTT A. · 2017 to 2020
$1.5M
Identification of Drug Targets for NF1R21NS060940 · NINDS · DARTMOUTH COLLEGE · PI SANCHEZ, YOLANDA · 2009 to 2010
$388k
Council of Scientific and Industrial Research, India 09/087(0972)/2019-EMR-IDepartment of Biotechnology, Ministry of Science and Technology, India BT/PR43050/BRB/10/1992/2021Department of Science and Technology, Ministry of Science and Technology, India DST/INSPIRE/03/2014/003008-IF150117NIGMS NIH HHS P20 GM113132NIGMS NIH HHS R01 GM122846NINDS NIH HHS R01 NS095411NINDS NIH HHS R21 NS060940
6 · The paper itself

Abstract

The 14-3-3 family of proteins are conserved across eukaryotes and serve myriad important regulatory functions in the cell. Homo- and hetero-dimers of these proteins mainly recognize their ligands via conserved motifs to modulate the localization and functions of those effector ligands. In most of the genetic backgrounds of Saccharomyces cerevisiae, disruption of both 14-3-3 homologs (Bmh1 and Bmh2) are either lethal or cells survive with severe growth defects, including gross chromosomal missegregation and prolonged cell cycle arrest. To elucidate their contributions to chromosome segregation, in this work, we investigated their centromere- and kinetochore-related functions of Bmh1 and Bmh2. Analysis of appropriate deletion mutants shows that Bmh isoforms have cumulative and non-shared isoform-specific contributions in maintaining the proper integrity of the kinetochore ensemble. Consequently, Bmh mutant cells exhibited perturbations in kinetochore-microtubule (KT-MT) dynamics, characterized by kinetochore declustering, mis-localization of kinetochore proteins and Mad2-mediated transient G2/M arrest. These defects also caused an asynchronous chromosome congression in bmh mutants during metaphase. In summary, this report advances the knowledge on contributions of budding yeast 14-3-3 proteins in chromosome segregation by demonstrating their roles in kinetochore integrity and chromosome congression.

Indexed as

14-3-3 ProteinsChromosome SegregationKinetochoresMitosisSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsChromosomes, FungalMicrotubules14-3-3 ProteinsSaccharomyces cerevisiae Proteins14-3-3 proteinsBmh1Bmh2Budding yeastChromosome congressionChromosome segregationKinetochore

Identifiers

PMID38988319
PMCPMC11698032

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.