Evidence map›Paper›PMID 38987854›Full record

ArticleBiology of sex differences2024

MicroRNA-21 modulates brown adipose tissue adipogenesis and thermogenesis in a mouse model of polycystic ovary syndrome.

Samar Rezq, Alexandra M Huffman, Jelina Basnet, Amira E Alsemeh, Jussara M do Carmo, Licy L Yanes Cardozo, Damian G Romero

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology of sex differences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Samar RezqDepartment of Pharmacology and Toxicology, University of Mississippi Medical Center, 2500 N. State Street, Jackson, MS, 39216, USA. srezq@umc.edu.
Alexandra M HuffmanWomen's Health Research Center, University of Mississippi Medical Center, 2500 N. State Street, Jackson, MS, 39216, USA.
Jelina BasnetDepartment of Pharmacology and Toxicology, University of Mississippi Medical Center, 2500 N. State Street, Jackson, MS, 39216, USA.
Amira E AlsemehDepartment of Anatomy, Histology, and Embryology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Jussara M do CarmoDepartment of Physiology and Biophysics, University of Mississippi Medical Center, 2500 N. State Street, Jackson, MS, 39216, USA.
Licy L Yanes CardozoDepartment of Pharmacology and Toxicology, University of Mississippi Medical Center, 2500 N. State Street, Jackson, MS, 39216, USA.
Damian G RomeroDepartment of Pharmacology and Toxicology, University of Mississippi Medical Center, 2500 N. State Street, Jackson, MS, 39216, USA. dromero@umc.edu.ORCID 0000-0002-7268-9690

Funding

STRUCTURAL VASCULAR ADAPTATION OF THE MICROCIRCULATIONP01HL051971 · NHLBI · UNIVERSITY OF MISSISSIPPI MEDICAL CENTER · PI RECKELHOFF, JANE F · 1993 to 2018
$39.4M
Investigator Development CoreP50MD017338 · NIMHD · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI BASKIN, MONICA L. · 2021 to 2025
$27.7M
Role of obesity in preeclamptic pregnancy.P20GM121334 · NIGMS · UNIVERSITY OF MISSISSIPPI MED CTR · PI Pier Paolo Claudio, Babbette LaMarca · 2017 to 2026
$26.4M
The role of leptin in autoimmune-associated hypertensionP20GM104357 · NIGMS · UNIVERSITY OF MISSISSIPPI MED CTR · PI HALL, JOHN E · 2013 to 2022
$23.4M
Pilot Projects ProgramP30GM149404 · NIGMS · UNIVERSITY OF MISSISSIPPI MED CTR · PI John E Hall · 2023 to 2026
$6.3M
Role of obesity in blood pressure regulation and insulin resistance in Polycystic Ovary SyndromeR01HL171494 · NHLBI · UNIVERSITY OF MISSISSIPPI MED CTR · PI LICY LORENA YANES CARDOZO · 2024 to 2026
$2.0M
Adrenal cell ATP1A1 mutations and mechanisms of aldosterone biosynthesisR01HL144847 · NHLBI · UNIVERSITY OF MISSISSIPPI MED CTR · PI GOMEZ-SANCHEZ, CELSO ENRIQUE · 2019 to 2022
$1.3M
Long-term consequences of parental obesity on developmental programming of cardiorenal diseases in offspringR01DK121411 · NIDDK · UNIVERSITY OF MISSISSIPPI MED CTR · PI DO CARMO, JUSSARA M. · 2019 to 2021
$818k
Role of microRNA_21 in acetaminophen-induced acute liver failureR21DK113500 · NIDDK · UNIVERSITY OF MISSISSIPPI MED CTR · PI ROMERO, DAMIAN G. · 2017 to 2019
$580k
American Heart Association 24PRE1200831American Heart Association 903804NHLBI NIH HHS P01 HL051971NHLBI NIH HHS P01HL51971NHLBI NIH HHS R01 HL144847NHLBI NIH HHS R01HL144847NHLBI NIH HHS R01 HL171494NHLBI NIH HHS R01HL171494NHLBI NIH HHS R0HL1630376NIDDK NIH HHS R01 DK121411NIDDK NIH HHS R01DK121411NIDDK NIH HHS R21 DK113500NIDDK NIH HHS R21DK113500NIGMS NIH HHS P20 GM104357NIGMS NIH HHS P20GM104357NIGMS NIH HHS P20 GM121334NIGMS NIH HHS P20GM121334NIGMS NIH HHS P30 GM149404NIGMS NIH HHS P30GM149404NIGMS NIH HHS P50MD017338
6 · The paper itself

Abstract

backgroundPolycystic ovary syndrome (PCOS), the most common endocrine disorder in premenopausal women, is associated with increased obesity, hyperandrogenism, and altered brown adipose tissue (BAT) thermogenesis. MicroRNAs play critical functions in brown adipocyte differentiation and maintenance. We aim to study the role of microRNA-21 (miR-21) in altered energy homeostasis and BAT thermogenesis in a PCOS mouse model of peripubertal androgen exposure.

methodsThree-week-old miR-21 knockout (miR21KO) or wild-type (WT) female mice were treated with dihydrotestosterone (DHT) or vehicle for 90 days. Body composition was determined by EchoMRI. Energy expenditure (EE), oxygen consumption (VO2), carbon dioxide production (VCO2), and respiratory exchange ratio (RER) were measured by indirect calorimetry. Androgen receptor (AR), and markers of adipogenesis, de novo lipogenesis, angiogenesis, extracellular matrix remodeling, and thermogenesis were quantified by RT-qPCR and/or Western-blot.

resultsMiR-21 ablation attenuated DHT-mediated increase in body weight while having no effect on fat or BAT mass. MiR-21 ablation attenuated DHT-mediated BAT AR upregulation. MiR-21 ablation did not alter EE; however, miR21KO DHT-treated mice have reduced VO2, VCO2, and RER. MiR-21 ablation reversed DHT-mediated decrease in food intake and increase in sleep time. MiR-21 ablation decreased some adipogenesis (Adipoq, Pparγ, and Cebpβ) and extracellular matrix remodeling (Mmp-9 and Timp-1) markers expression in DHT-treated mice. MiR-21 ablation abolished DHT-mediated increases in thermogenesis markers Cpt1a and Cpt1b, while decreasing CIDE-A expression.

conclusionsOur findings suggest that BAT miR-21 may play a role in regulating DHT-mediated thermogenic dysfunction in PCOS. Modulation of BAT miR-21 levels could be a novel therapeutic approach for the treatment of PCOS-associated metabolic derangements.

Indexed as

AdipogenesisAdipose Tissue, BrownDisease Models, AnimalMice, KnockoutMicroRNAsPolycystic Ovary SyndromeThermogenesisAnimalsDihydrotestosteroneFemaleMiceMice, Inbred C57BLReceptors, AndrogenDihydrotestosteroneMicroRNAsMIRN21 microRNA, mouseReceptors, AndrogenAdipogenesisBrown adipose tissueEnergy expenditurePolycystic ovary syndromeThermogenesis

Identifiers

PMID38987854
PMCPMC11238487

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.