Evidence map›Paper›PMID 38986535›Full record

ArticleStem cells translational medicine2024

Requirement of Pdgfrα+ cells for calvarial bone repair.

Xin Xing, Zhao Li, Jiajia Xu, Austin Z Chen, Mary Archer, Yiyun Wang, Mingxin Xu, Ziyi Wang, Manyu Zhu, Qizhi Qin and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells translational medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Xin XingDepartment of Pathology, Johns Hopkins University, Baltimore, MD 21205, United States.ORCID 0000-0001-6417-0664
Zhao LiDepartment of Pathology, Johns Hopkins University, Baltimore, MD 21205, United States.
Jiajia XuDepartment of Pathology, Johns Hopkins University, Baltimore, MD 21205, United States.
Austin Z ChenDepartment of Pathology, Johns Hopkins University, Baltimore, MD 21205, United States.
Mary ArcherDepartment of Pathology, Johns Hopkins University, Baltimore, MD 21205, United States.
Yiyun WangDepartment of Pathology, Johns Hopkins University, Baltimore, MD 21205, United States.
Mingxin XuDepartment of Pathology, Johns Hopkins University, Baltimore, MD 21205, United States.
Ziyi WangDepartment of Pathology, Johns Hopkins University, Baltimore, MD 21205, United States.
Manyu ZhuDepartment of Pathology, Johns Hopkins University, Baltimore, MD 21205, United States.
Qizhi QinDepartment of Pathology, Johns Hopkins University, Baltimore, MD 21205, United States.
Neelima ThottappillilDepartment of Pathology, Johns Hopkins University, Baltimore, MD 21205, United States.ORCID 0000-0003-3973-7117
Myles ZhouDepartment of Pathology, Johns Hopkins University, Baltimore, MD 21205, United States.
Aaron W JamesDepartment of Pathology, Johns Hopkins University, Baltimore, MD 21205, United States.ORCID 0000-0002-2002-622X

Funding

Skeleton and Joint Degeneration with AgingP01AG066603 · NIA · JOHNS HOPKINS UNIVERSITY · PI CAO, XU · 2021 to 2025
$9.2M
Impact of peripheral nerves on mesenchymal cell fate in extremity traumaR01AR079171 · NIAMS · UT SOUTHWESTERN MEDICAL CENTER · PI JAMES, AARON W, LEVI, BENJAMIN · 2021 to 2024
$3.0M
Biostimulatory nanofiber-hydrogel composite for soft tissue remodelingR01DE031488 · NIDCR · JOHNS HOPKINS UNIVERSITY · PI AARON W JAMES, Hai-Quan Mao · 2022 to 2026
$2.8M
Neuronal Regulation of Skeletal Development and RepairR01DE031028 · NIDCR · UNIVERSITY OF MARYLAND BALTIMORE · PI CLEMENS, THOMAS L, JAMES, AARON W · 2021 to 2025
$2.3M
NIAMS NIH HHS R01 AR079171NIA NIH HHS P01 AG066603NIDCR NIH HHS R01 DE031028NIDCR NIH HHS R01 DE031488
6 · The paper itself

Abstract

Platelet-derived growth factor receptor α (PDGFRα) is often considered as a general marker of mesenchymal cells and fibroblasts, but also shows expression in a portion of osteoprogenitor cells. Within the skeleton, Pdgfrα+ mesenchymal cells have been identified in bone marrow and periosteum of long bones, where they play a crucial role in participating in fracture repair. A similar examination of Pdgfrα+ cells in calvarial bone healing has not been examined. Here, we utilize Pdgfrα-CreERTM;mT/mG reporter animals to examine the contribution of Pdgfrα+ mesenchymal cells to calvarial bone repair through histology and single-cell RNA sequencing (scRNA-Seq). Results showed that Pdgfrα+ mesenchymal cells are present in several cell clusters by scRNA-Seq, and by histology a dramatic increase in Pdgfrα+ cells populated the defect site at early timepoints to give rise to healed bone tissue overtime. Notably, diphtheria toxin-mediated ablation of Pdgfrα reporter+ cells resulted in significantly impaired calvarial bone healing. Our findings suggest that Pdgfrα-expressing cells within the calvarial niche play a critical role in the process of calvarial bone repair.

Indexed as

Receptor, Platelet-Derived Growth Factor alphaSkullAnimalsBone RegenerationMesenchymal Stem CellsMiceReceptor, Platelet-Derived Growth Factor alphabone healingbone repaircalvarial bonemesenchymal cellsplatelet-derived growth factor receptorsingle-cell RNA sequencingskeletal cells

Identifiers

PMID38986535
PMCPMC11328938

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.