Evidence map›Paper›PMID 38985267›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Protocol for Generating Arabidopsis thaliana Cell Suspension Cultures Under Different Light Conditions.

Sara Hernández-Castellano, Rosa Ma Galaz-Ávalos, Víctor M Loyola-Vargas, Clelia De-la-Peña

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Sara Hernández-CastellanoUnidad de Biotecnología, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, México.
Rosa Ma Galaz-ÁvalosUnidad de Biología Integrativa, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, México.
Víctor M Loyola-VargasUnidad de Biología Integrativa, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, México.
Clelia De-la-PeñaUnidad de Biotecnología, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, México. clelia@cicy.mx.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plant cell suspension cultures (PCSCs) are in vitro-cultured cells that can divide indefinitely in a sterile growth medium. These PCSCs can be derived from various plant tissues, such as the root, stem, leaves, or seeds, and are maintained in a suitable culture medium containing nutrients, vitamins, hormones, and other essential components necessary for their growth. PCSCs have extensive applications in biotechnology, particularly in producing pharmaceutical and chemical compounds. This chapter presents a protocol for generating cell lines from Arabidopsis thaliana root callus under different light conditions, which can be used to investigate the effects of light on plant cell growth and development. The protocol described in this chapter is a valuable tool for researchers interested in utilizing PCSCs in their studies.

Indexed as

ArabidopsisCell Culture TechniquesLightCells, CulturedCulture MediaPlant RootsCulture MediaArabidopsis thalianaBiotechnologyIn Vitro cultureLight conditionsPlant cell lines

Identifiers

PMID38985267

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.