Evidence map›Paper›PMID 38984587›Full record

ArticleBiology open2024

Safety assessment of rat embryonic fraction for in vivo regenerative therapy.

Sivarama Prasad Darsi, Somorita Baishya, Veerababu Nagati, Kala Kumar Bharani, Satyanarayana Swamy Cheekatla, Sujesh Kumar Darsi, Adi Reddy Kamireddy, Ram Reddy Barra, Ashok Kumar Devarasetti, Sreedhar Surampudi and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology open, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Sivarama Prasad DarsiDepartment of Biotechnology, School of Life Sciences, Gitam University, Visakhapatnam, AP, India530045.ORCID 0000-0001-9986-4199
Somorita BaishyaDepartment of Biochemistry, University of Hyderabad, Hyderabad, TG, India500046.
Veerababu NagatiDepartment of Biochemistry, University of Hyderabad, Hyderabad, TG, India500046.
Kala Kumar BharaniDepartment of Veterinary Pharmacology and Toxicology, P.V. Narasimha Rao University of Veterinary Sciences, Rajendra Nagar, TG, India500030.
Satyanarayana Swamy CheekatlaDepartment of Biotechnology, School of Life Sciences, Gitam University, Visakhapatnam, AP, India530045.
Sujesh Kumar DarsiDepartment of General Medicine, ESI Corporation, Gunadala, Vijayawada, AP, India520004.
Adi Reddy KamireddyDepartment of Internal Medicine, Banner Health Center, Maricopa, AZ, USA85138.
Ram Reddy BarraDepartment of Physiology, Apollo Institute of Medical Sciences and Research, Hyderabad, TG 500090, India.
Ashok Kumar DevarasettiDepartment of Veterinary Biochemistry, P.V. Narasimha Rao University of Veterinary Sciences, Mamnoor, Warangal, TG, India506166.
Sreedhar SurampudiDepartment of Biochemistry, Aware College of Medical Lab Technology, Bairamalguda, Hyderabad 500035, India.
Jayaram Reddy SingireddyDepartment of Urology, Hyderabad Kidney & Laparoscopic Centre, Malakpet, Hyderabad, TG 500036, India.
Siva Kumar KandulaDepartment of Biotechnology, School of Life Sciences, Gitam University, Visakhapatnam, AP, India530045.
Anil Kumar PasupulatiDepartment of Biochemistry, University of Hyderabad, Hyderabad, TG, India500046.ORCID 0000-0001-9467-7650

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Regenerative therapy is considered a novel option for treating various diseases, whereas a developing embryo is a prime source of molecules that help repair diseased tissue and organs. Organoid culture studies also confirmed the inherent biological functions of several embryonic factors. However, the in vivo safety and efficacy of embryonic protein fraction (EPF) were not validated. In this study, we investigated the effectiveness of EPF on healthy adult rats. We obtained embryos from Sprague-Dawley (SD) female rats of E14, E16, and E19 embryonic days and collected protein lysate. This lysate was administered intravenously into adult SD rats on sequential days. We collected blood and performed hematological and biochemical parameters of rats that received EPF. C-reactive protein levels, interleukin-6, blood glucose levels, serum creatinine, blood urea, total leucocyte counts, and % of neutrophils and lymphocytes were comparable between rats receiving EPF and saline. Histological examination of rats' tissues administered with EPF is devoid of abnormalities. Our study revealed that intravenous administration of EPF to healthy adult rats showed that EPF is non-immunogenic, non-inflammatory, non-tumorigenic, and safe for in vivo applications. Our analysis suggests that EPF or its components could be recommended for validating its therapeutic abilities in organ regenerative therapy.

Indexed as

Regenerative MedicineAnimalsEmbryo, MammalianFemaleRatsRats, Sprague-DawleyEmbryonic protein fractionImmunogenicityProteomic analysisRegenerative therapyTumorigenicity

Identifiers

PMID38984587
PMCPMC11360137

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.