Evidence map›Paper›PMID 38980520›Full record

ArticleMetabolomics : Official journal of the Metabolomic Society2024

Dispel some mist on circulating biopterins: measurement, physiological interval and pathophysiological implication.

Hai-Bo Wang, Xuan Xiao, Wen Dai, Yan Cui, Wan-Man Li, Rui Peng, Liu Hu, Shao-Ting Wang

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In one paragraph

Article in Metabolomics : Official journal of the Metabolomic Society, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
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1citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Hai-Bo Wang *Department of Clinical Laboratory, Institute of Translational Medicine, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Xuan Xiao *Department of Clinical Laboratory, Institute of Translational Medicine, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Wen Dai *Department of Clinical Laboratory, Institute of Translational Medicine, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Yan Cui *Department of Clinical Laboratory, Institute of Translational Medicine, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Wan-Man Li *Department of Clinical Laboratory, Institute of Translational Medicine, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Rui PengDepartment of Clinical Laboratory, Institute of Translational Medicine, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Liu HuPhysical Examination Center, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Shao-Ting WangDepartment of Clinical Laboratory, Institute of Translational Medicine, Renmin Hospital of Wuhan University, Wuhan, 430060, China. shaotingw@whu.edu.cn.ORCID http://orcid.org/0000-0001-9976-0445

Funding

Fundamental Research Funds for the Central Universities 2042020kf0067Interdisciplinary Innovative Talents Foundation from Renmin Hospital of Wuhan University JCRCFZ-2022-001
6 · The paper itself

Abstract

BACKGROUND AND

aimsBiopterins, including tetrahydrobiopterin (BH4), dihydrobiopterin (BH2), and biopterin (B), were crucial enzyme cofactors in vivo. Despite their recognized clinical significance, there remain notable research gaps and controversies surrounding experimental outcomes. This study aims to clarify the biopterins-related issues, including analytical art, physiological intervals, and pathophysiological implications. MATERIALS AND

methodsA novel LC-MS/MS method was developed to comprehensively profile biopterins in plasma, utilizing chemical derivatization and cold-induced phase separation. Subsequently, apparently healthy individuals were enrolled to investigate the physiological ranges. And the relationships between biopterins and biochemical indicators were analyzed to explore the pathophysiological implications.

resultsThe developed method was validated as reliable for detecting biopterins across the entire physiological range. Timely anti-oxidation was found to be essential for accurate assessment of biopterins. The observed overall mean ± SDs levels were 3.51 ± 0.94, 1.54 ± 0.48, 2.45 ± 0.84 and 5.05 ± 1.14 ng/mL for BH4, BH2, BH4/BH2 and total biopterins. The status of biopterins showed interesting correlations with age, gender, hyperuricemia and overweight.

conclusionIn conjunction with proper anti-oxidation, the newly developed method enables accurate determination of biopterins status in plasma. The observed physiological intervals and pathophysiological implications provide fundamental yet inspiring support for further clinical researches.

Indexed as

BiopterinsTandem Mass SpectrometryAdultAgedBiomarkersChromatography, LiquidFemaleHumansMaleMiddle AgedYoung AdultBiomarkersBiopterinssapropterinBiopterinDihydrobiopterinMass spectrometryOxidative stressTetrahydrobiopterin

Identifiers

PMID38980520

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.