Evidence map›Paper›PMID 38980074›Full record

ArticlemSphere2024

Autoclaving is at least as effective as gamma irradiation for biotic clearing and intentional microbial recolonization of soil.

William L King, Emily M Grandinette, Olivia Trase, M Laura Rolon, Howard M Salis, Harlow Wood, Terrence H Bell

Abstract read
In one paragraph

Article in mSphere, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Article
  3. Tracing Soil COEnvironmental science & technology · 2026
    Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Survival and spread of engineeredApplied and environmental microbiology · 2025
    Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

William L KingDepartment of Plant Pathology and Environmental Microbiology, The Pennsylvania State University, University Park, Pennsylvania, USA.ORCID 0000-0001-7272-8242
Emily M GrandinetteDepartment of Plant Pathology and Environmental Microbiology, The Pennsylvania State University, University Park, Pennsylvania, USA.
Olivia TraseDepartment of Entomology, The Pennsylvania State University, University Park, Pennsylvania, USA.
M Laura RolonDepartment of Food Science, The Pennsylvania State University, University Park, Pennsylvania, USA.
Howard M SalisDepartment of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, Pennsylvania, USA.
Harlow WoodDepartment of Physical & Environmental Sciences, University of Toronto Scarborough, Toronto, Ontario, Canada.
Terrence H BellDepartment of Plant Pathology and Environmental Microbiology, The Pennsylvania State University, University Park, Pennsylvania, USA.ORCID 0000-0003-3603-7270

Funding

DARPA Bioreporters grant HR00111920021U.S. Department of Agriculture (USDA) 1016233
6 · The paper itself

Abstract

Sterilization is commonly used to remove or reduce the biotic constraints of a soil to allow recolonization by soil-dwelling organisms, with autoclaving and gamma irradiation being the most frequently used approaches. Many studies have characterized sterilization impacts on soil physicochemical properties, with gamma irradiation often described as the preferred approach, despite the lower cost and higher scalability of autoclaving. However, few studies have compared how sterilization techniques impact soil recolonization by microorganisms. Here, we compared how two sterilization approaches (autoclaving; gamma irradiation) and soil washing impacted microbial recolonization of soil from a diverse soil inoculum. Sterilization method had little impact on microbial alpha diversity across recolonized soils. For sterile soil regrowth microcosms, species richness and diversity were significantly reduced by autoclaving relative to gamma irradiation, particularly for fungi. There was no impact of sterilization method on bacterial composition in recolonized soils and minimal impact on fungal composition (

Indexed as

BacteriaFungiGamma RaysSoilSoil MicrobiologySterilizationBiodiversityHot TemperatureMicrobiotaSoilautoclavingbiotic-constraintsgamma-irradiationmicrobial recolonizationsoil microcosmsoil sterilization

Identifiers

PMID38980074
PMCPMC11288020

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.