Evidence map›Paper›PMID 38979669›Full record

ArticleAnimal models and experimental medicine2024

Ultra-high-performance liquid chromatography-quadrupole time-of-flight-mass spectrometry-characterized extract of Aerides odorata Lour alleviates paracetamol-induced hepatotoxicity in animal model evidenced by biochemical, molecular, and computational studies.

A M Abu Ahmed, Md Atiar Rahman, Farjana Sharmen, A S M Ali Reza, Md Shahidul Islam, Md Mamunur Rashid, Md Khalid Juhani Rafi, Tanvir Ahmed Siddiqui, Md Muzahid Ahmed Ezaj, Srabonti Saha and 2 more

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Article in Animal models and experimental medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

A M Abu AhmedDepartment of Genetic Engineering and Biotechnology, University of Chittagong, Chittagong, Bangladesh.
Md Atiar RahmanDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chittagong, Bangladesh.ORCID 0000-0002-4902-8923
Farjana SharmenDepartment of Genetic Engineering and Biotechnology, University of Chittagong, Chittagong, Bangladesh.
A S M Ali RezaDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chittagong, Bangladesh.
Md Shahidul IslamDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chittagong, Bangladesh.
Md Mamunur RashidDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chittagong, Bangladesh.
Md Khalid Juhani RafiDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chittagong, Bangladesh.
Tanvir Ahmed SiddiquiDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chittagong, Bangladesh.
Md Muzahid Ahmed EzajDepartment of Genetic Engineering and Biotechnology, University of Chittagong, Chittagong, Bangladesh.
Srabonti SahaDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chittagong, Bangladesh.
Md Nazim UddinInstitute of Food Science and Technology, Bangladesh Council of Scientific and Industrial Research, Dhaka, Bangladesh.
Walla AlelwaniDepartment of Biochemistry, College of Science, University of Jeddah, Jeddah, Saudi Arabia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMany kinds of orchids have significant health benefits although adequate research on their biological functions is yet to be carried out. This study investigated the paracetamol-induced liver damage-protecting effect of epiphytic Aerides odorata methanol extract (AODE).

methodsThe protective effects of AODE were studied by analyzing its effect on liver function parameters, messenger RNA (mRNA) expression, and tissue histopathological architecture. The results were confirmed by ligand-receptor interaction of molecular docking and multitarget interaction of network pharmacological analyses.

resultsAODE significantly (p < 0.05) minimized the dose-dependent increase in acid phosphatase, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, γ-glutamyl transferase, lactate dehydrogenase, and total bilirubin compared to the reference drug silymarin. Malondialdehyde level decreased, and the antioxidant genes catalase (CAT), superoxide dismutase (SOD), β-actin, paraoxonase-1 (PON1), and phosphofructokinase-1 (PFK-1) were upregulated in AODE-treated paracetamol-intoxicated rats. A total of 376 compounds comprising phenols and flavonoids were identified using ultra-high-performance liquid chromatography-quadrupole time-of-flight-mass spectrometry (UPLC-qTOF-MS). The online toxicity assessment using SwissADME and admetSAR exhibited drug-like, nontoxic, and potential pharmacological properties. Additionally, in silico analysis showed that isoacteoside, one of the identified compounds, exhibited the best docking score (-11.42) with the liver protein human pituitary adenylate cyclase-1 (Protein Data Bank ID: 3N94). Furthermore, network pharmacology analysis identified the top 10 hub genes, namely AKT1 (protein kinase B), CTNNB1 (catenin beta-1), SRC (proto-oncogene c-Src), TNF (tumor necrosis factor), EGFR (epidermal growth factor receptor), HSP90AA1 (heat shock protein 90α), MAPK3 (mitogen-activated protein kinase 3), STAT3 (signal transducer and activator of transcription 3), CASP3 (caspase protein), and ESR1 (estrogen receptor 1), which are responsible for hepatoprotective activity.

conclusionThe findings demonstrate that AODE could be a novel hepatoprotective target in drug-induced liver damage with a further single compound-based animal study.

Indexed as

AcetaminophenChemical and Drug Induced Liver InjuryMolecular Docking SimulationPlant ExtractsAnimalsChromatography, High Pressure LiquidDisease Models, AnimalLiverMaleMass SpectrometryNetwork PharmacologyOrchidaceaeRatsRats, WistarAcetaminophenPlant ExtractsAerides odorataanimal modelmRNA expressionPCM‐induced hepatotoxicityUPLC‐qTOF‐MS

Identifiers

PMID38979669
PMCPMC11369029

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.