Methods for negating the impact of zinc contamination to allow characterization of positive allosteric modulators of glycine receptors.

Casey I Gallagher, David P Bishop, Thomas E Lockwood, Tristan Rawling, Robert J Vandenberg

Abstract read

In one paragraph

Article in Frontiers in molecular neuroscience, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Structure of a pH-sensitive pentameric ligand-gated ion channel from the Sarcoptes scabies mite.Nature communications · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Casey I GallagherDiscipline of Pharmacology, School of Medical Sciences, University of Sydney, Sydney, NSW, Australia.

David P BishopHyphenated Mass Spectrometry Laboratory, Faculty of Science, University of Technology Sydney, Broadway, NSW, Australia.

Thomas E LockwoodHyphenated Mass Spectrometry Laboratory, Faculty of Science, University of Technology Sydney, Broadway, NSW, Australia.

Tristan RawlingSchool of Mathematical and Physical Sciences, Faculty of Science, The University of Technology Sydney, Sydney, NSW, Australia.

Robert J VandenbergDiscipline of Pharmacology, School of Medical Sciences, University of Sydney, Sydney, NSW, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Zinc is a ubiquitous contaminant in many buffers, purified products and common labware that has previously been suggested to impact on the results of functional GlyR studies and may inadvertently cause the effectiveness of some GlyR modulators to be over-estimated. This could greatly impact the assessment of potential drug-candidates and contribute to the reduced effectiveness of compounds that reach clinical stages. This is especially true for GlyR modulators being developed for pain therapeutics due to the changes in spinal zinc concentrations that have been observed during chronic pain conditions. In this study we use two-electrode voltage clamp electrophysiology to evaluate the metal chelators tricine and Ca-EDTA, and show that tricine produces inhibitory effects at GlyRα

Indexed as

bioactive lipidchelatordrug developmentglycine receptorpositive allosteric modulatortricinezinc

Identifiers

PMID38979476

DOI10.3389/fnmol.2024.1392715

PMCPMC11228362

What OpenQuestion holds

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LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.