Evidence map›Paper›PMID 38979370›Full record

ArticlebioRxiv : the preprint server for biology2024

Model-guided design of microRNA-based gene circuits supports precise dosage of transgenic cargoes into diverse primary cells.

Kasey S Love, Christopher P Johnstone, Emma L Peterman, Stephanie Gaglione, Kate E Galloway

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Kasey S LoveDepartment of Biological Engineering, MIT, 25 Ames St., Cambridge, MA 02139, USA.ORCID 0000-0001-7544-0340
Christopher P JohnstoneDepartment of Chemical Engineering, MIT, 25 Ames St., Cambridge, MA 02139, USA.ORCID 0000-0002-7255-0218
Emma L PetermanDepartment of Chemical Engineering, MIT, 25 Ames St., Cambridge, MA 02139, USA.ORCID 0000-0001-7402-576X
Stephanie GaglioneDepartment of Chemical Engineering, MIT, 25 Ames St., Cambridge, MA 02139, USA.
Kate E GallowayDepartment of Chemical Engineering, MIT, 25 Ames St., Cambridge, MA 02139, USA.ORCID 0000-0001-7416-3193

Funding

Multiscale tools and approaches for understanding and engineering cell-fate transitionsR35GM143033 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI GALLOWAY, KATE ELIZABETH · 2021 to 2025
$1.9M
NIGMS NIH HHS R35 GM143033
6 · The paper itself

Abstract

To realize the potential of engineered cells in therapeutic applications, transgenes must be expressed within the window of therapeutic efficacy. Differences in copy number and other sources of extrinsic noise generate variance in transgene expression and limit the performance of synthetic gene circuits. In a therapeutic context, supraphysiological expression of transgenes can compromise engineered phenotypes and lead to toxicity. To ensure a narrow range of transgene expression, we design and characterize

Identifiers

PMID38979370
PMCPMC11230401

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.