Evidence map›Paper›PMID 38979301›Full record

ArticlebioRxiv : the preprint server for biology2024

Directed evolution of bacteriophages: impacts of prolific prophage.

Tracey Lee Peters, Jacob Schow, Emma Spencer, J T Van Leuven, Holly Wichman, Craig Miller

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Tracey Lee PetersInstitute for Modeling Collaboration and Innovation, University of Idaho, Moscow, Idaho 83844, USA.ORCID 0000-0003-4644-8743
Jacob SchowDepartment of Biological Sciences, University of Idaho, Moscow, Idaho 83844, USA.
Emma SpencerDepartment of Biological Sciences, University of Idaho, Moscow, Idaho 83844, USA.
J T Van LeuvenDepartment of Animal, Veterinary and Food Sciences, University of Idaho, Moscow, Idaho 83844, USA.
Holly WichmanInstitute for Modeling Collaboration and Innovation, University of Idaho, Moscow, Idaho 83844, USA.
Craig MillerDepartment of Biological Sciences, University of Idaho, Moscow, Idaho 83844, USA.

Funding

Sequence-structure-function relationships in human visual photopigmentsP20GM104420 · NIGMS · UNIVERSITY OF IDAHO · PI MILLER, CRAIG R · 2015 to 2024
$22.9M
Patterns of Adaptive EvolutionR01GM076040 · NIGMS · UNIVERSITY OF IDAHO · PI MILLER, CRAIG R, WICHMAN, HOLLY A · 2006 to 2021
$3.6M
NIGMS NIH HHS P20 GM104420NIGMS NIH HHS R01 GM076040
6 · The paper itself

Abstract

Various directed evolution methods exist that seek to procure bacteriophages with expanded host ranges, typically targeting phage-resistant or non-permissive bacterial hosts. The general premise of these methods is to propagate phage on multiple bacterial hosts, pool the lysate, and repeat the propagation process until phage(s) can form plaques on the target host(s). In theory, this propagation process produces a phage lysate that contains input phages and their evolved phage progeny. However, in practice, this phage lysate can also include prophages originating from bacterial hosts. Here we describe our experience implementing one directed evolution method, the Appelmans protocol, to study phage evolution in the

Indexed as

Appelmansbacteriophagedirected evolutionhost rangeprophagePseudomonas aeruginosa

Identifiers

PMID38979301
PMCPMC11230397

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.