Evidence map›Paper›PMID 38979230›Full record

ArticlebioRxiv : the preprint server for biology2024

An efficient method for immortalizing mouse embryonic fibroblasts.

Srisathya Srinivasan, Hsin-Yi Henry Ho

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors.

Srisathya SrinivasanDepartment of Cell Biology and Human Anatomy, University of California, Davis, Davis, USA.ORCID 0000-0002-4502-5063
Hsin-Yi Henry HoDepartment of Cell Biology and Human Anatomy, University of California, Davis, Davis, USA.ORCID 0000-0002-8780-7864

Funding

Function and regulatory mechanisms of the Wnt5a-Ror morphogenetic pathwayR35GM144341 · NIGMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI Hsin-Yi Henry Ho · 2022 to 2026
$2.2M
Deciphering Wnt-Ror signaling in cytoskeletal regulation and tissue shape controlR35GM119574 · NIGMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI HO, HSIN-YI HENRY · 2016 to 2021
$2.0M
NIGMS NIH HHS R35 GM119574NIGMS NIH HHS R35 GM144341
6 · The paper itself

Abstract

Mouse embryonic fibroblasts (MEFs) derived from genetically modified mice are a valuable resource for studying gene function and regulation. The MEF system can also be combined with rescue studies to characterize the function of mutant genes/proteins, such as disease-causing variants. However, primary MEFs undergo senescence soon after isolation and passaging, making long-term genetic manipulations difficult. Previously described methods for MEF immortalization are often inefficient or alter the physiological properties of the cells. Here, we describe an optimized protocol for immortalizing MEFs via CRISPR-mediated deletion of the

Indexed as

CRISPR/Cas9immortalized mouse embryonic fibroblasts (iMEFs)MEF immortalizationMEF isolationMouse embryonic fibroblasts (MEF)Tp53 knockout

Identifiers

PMID38979230
PMCPMC11230376

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.