ArticleCritical care (London, England)2024
Clinical application of targeted next-generation sequencing in severe pneumonia: a retrospective review.
Article in Critical care (London, England), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed.
- Rapid diagnosis ofExperimental and therapeutic medicine · 2026Article
- Development and Initial Analytical Evaluation of a Multiplex PCR-Based Targeted NGS Assay for Expanded Viral Detection in Clinical Respiratory Specimens.Diagnostics (Basel, Switzerland) · 2026Article
- Clinical Utility of Metagenomic Next-Generation Sequencing in Adult Patients with Fever of Unknown Origin: A Retrospective Real-World Study.Journal of clinical medicine · 2026Article
- [Application of metagenomic next-generation sequencing in the pathogen spectrum analysis of suspected infections in neonatal blood and cerebrospinal fluid].Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics · 2026Article
- Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis.Respiratory research · 2026Article
- Epidemiological and pathogen characteristics of symptomatic male reproductive tract infections in Hunan province: retrospective study.Scientific reports · 2026Article
- Quantitative interpretation models for targeted next-generation sequencing in lower respiratory tract infections: a multicenter prospective study.Respiratory research · 2026Article
- Association between normalizedTranslational pediatrics · 2026Article
- Broad-spectrum targeted next-generation sequencing: is it ready for routine deployment in intensive care units for severe pneumonia?Critical care (London, England) · 2026Article
- Bordetella pertussis re-emergence - NGS-based RPTM analysis reveals the atypical clinical features of children.BMC infectious diseases · 2026Article
- Pathogen landscape and synergistic interactions in pediatric ARIs: implications for broad-spectrum surveillance via targeted NGS.Microbiology spectrum · 2026Article
- Targeted next-generation sequencing of bronchoalveolar lavage fluid for pathogen detection in connective tissue disease-related pulmonary infections.Microbiology spectrum · 2026Article
- A Pilot Study ofDiagnostics (Basel, Switzerland) · 2026Article
- Research progress on the current status of respiratory pathogen infections and their detection methods.Frontiers in microbiology · 2026Review
- Clinical and Microbiological Characteristics of Pulmonary Aspergillosis in Elderly vs Non-Elderly Patients: A tNGS-Based Retrospective Study.Infection and drug resistance · 2026Article
- No culture? No problem: Clinical utility and pitfalls of non-culture diagnostics for pneumococcal parapneumonic effusions.Frontiers in medicine · 2026Review
- Article
- Early diagnosis and therapy of Chlamydia psittaci pneumonia: insights from chest CT, tNGS, and omadacycline in 34 cases.Frontiers in medicine · 2026Article
- A diagnostic performance study of multiplex polymerase chain reaction-based targeted next-generation sequencing for the accurate identification ofFrontiers in cellular and infection microbiology · 2026Article
- Clinical diagnostic value of targeted next generation sequencing for lower respiratory tract infection: a retrospective study.Frontiers in cellular and infection microbiology · 2026Article
4 more citing papers are in PubMed but not listed here.
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25 authors.
Funding
Abstract
backgroundThe precise identification of the underlying causes of infectious diseases, such as severe pneumonia, is essential, and the development of next-generation sequencing (NGS) has enhanced the effectiveness of pathogen detection. However, there is limited information on the systematic assessment of the clinical use of targeted next-generation sequencing (tNGS) in cases of severe pneumonia.
methodsA retrospective analysis was conducted on 130 patients with severe pneumonia treated in the ICU from June 2022 to June 2023. The consistency of the results of tNGS, metagenomics next-generation sequencing (mNGS), and culture with the clinical diagnosis was evaluated. Additionally, the results for pathogens detected by tNGS were compared with those of culture, mNGS, and quantitative reverse transcription PCR (RT-qPCR). To evaluate the efficacy of monitoring severe pneumonia, five patients with complicated infections were selected for tNGS microbiological surveillance. The tNGS and culture drug sensitisation results were then compared.
resultsThe tNGS results for the analysis of the 130 patients showed a concordance rate of over 70% with clinical diagnostic results. The detection of pathogenic microorganisms using tNGS was in agreement with the results of culture, mNGS, and RT-qPCR. Furthermore, the tNGS results for pathogens in the five patients monitored for complicated infections of severe pneumonia were consistent with the culture and imaging test results during treatment. The tNGS drug resistance results were in line with the drug sensitivity results in approximately 65% of the cases.
conclusionsThe application of tNGS highlights its promise and significance in assessing the effectiveness of clinical interventions and providing guidance for anti-infection therapies for severe pneumonia.
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