Diacylglycerol Signaling in Retinal Ganglion Cells.

Ishan Chaplot, Isabella Moceri, Maria D Cabrera Gonzalez, Sanjoy K Bhattacharya

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Ishan ChaplotBascom Palmer Eye Institute, Miami Integrative Metabolomics Research Center, University of Miami, Miami, FL, USA.

Isabella MoceriBascom Palmer Eye Institute, Miami Integrative Metabolomics Research Center, Miller School of Medicine at University of Miami, Miami, FL, USA.

Maria D Cabrera GonzalezDepartment of Ophthalmology, Bascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, USA.

Sanjoy K BhattacharyaMiami Integrative Metabolomics Research Center, Miami, FL, USA. sbhattacharya@med.miami.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

With impaired retinal ganglion cell (RGC) function and eventual RGC death, there is a heightened risk of experiencing glaucoma-induced blindness or other optic neuropathies. Poor RGC efficiency leads to limited transmission of visual signals between the retina and the brain by RGC axons. Increased focus on studying lipid messengers found in neurons such as endocannabinoids (eCBs) has importance due to their potential axonal pathway regenerative properties. 2-Arachidonoylglycerol (2-AG), a common eCB, is synthesized from an sn-1 hydrolysis reaction between diacylglycerol (DAG) and diacylglycerol lipase (DAGL). Examination of DAG production allows for future downstream analysis in relation to DAGL functionality. Here, we describe protocol guidelines for extracting RGCs from mouse retinas and subsequent mass spectrometry analysis of the DAG content present within the RGCs.

Indexed as

DiglyceridesRetinal Ganglion CellsSignal TransductionAnimalsArachidonic AcidsEndocannabinoidsGlyceridesLipoprotein LipaseMass SpectrometryMiceRetinaArachidonic AcidsDiglyceridesEndocannabinoidsGlyceridesglyceryl 2-arachidonateLipoprotein LipaseAxon regenerationDiacylglycerolDiacylglycerol signalingMass spectrometryRetinal ganglion cells

Identifiers

PMID38977600

DOI10.1016/j.isci.2023.106814

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.