Method to Assess the Intracellular Fate and Bioavailability of Clusterin Using Live Cell Confocal Microscopy Imaging.

Avinash Soundararajan, Padmanabhan Paranji Pattabiraman

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Avinash SoundararajanDepartment of Ophthalmology, Glick Eye Institute, Indiana University School of Medicine, Indianapolis, IN, USA.

Padmanabhan Paranji PattabiramanDepartment of Ophthalmology, Glick Eye Institute, Indiana University School of Medicine, Indianapolis, IN, USA. ppattabi@iu.edu.

Funding

Role of Clusterin in Aqueous Humor Outflow PhysiologyR01EY029320 · NEI · INDIANA UNIVERSITY INDIANAPOLIS · PI PATTABIRAMAN, PADMANABHAN PARANJI · 2019 to 2023

$2.0M

Transcriptional control of lipid biogenesis in trabecular meshwork and intraocular pressure regulationR01EY035412 · NEI · INDIANA UNIVERSITY INDIANAPOLIS · PI Padmanabhan Paranji Pattabiraman · 2024 to 2026

$1.5M

NEI NIH HHS R01 EY029320NEI NIH HHS R01 EY035412

6 · The paper itself

Abstract

Clusterin, also known as apolipoprotein J, is an ATP-independent holdase chaperone protein. Clusterin is involved in various functions including protein quality control and lipid transport. Though clusterin is secreted upon stress, the intracellular fate of clusterin after a stress response is not well understood. The protocol described here utilizes clusterin tagged to fluorescent proteins like green fluorescent protein and red fluorescent protein to understand the intracellular fate of clusterin.

Indexed as

ClusterinMicroscopy, ConfocalAnimalsGreen Fluorescent ProteinsHumansLuminescent ProteinsRed Fluorescent ProteinClusterinGreen Fluorescent ProteinsLuminescent ProteinsRed Fluorescent ProteinClusterinGreen fluorescent proteinHuman trabecular meshworkRed fluorescent proteinSecretory proteinSignal peptide

Identifiers

PMID38977596

DOI10.1016/j.tibs.2021.01.005

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.