Evidence map›Paper›PMID 38977575›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Genome Editing to Produce Knockout Mutations of Seed Dormancy Genes in Wheat.

Fumitaka Abe, Yoko Kamiya, Yuji Ishida, Hiroshi Hisano, Kanako Kawaura, Toshihiko Komari, Kazuhiro Sato

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Fumitaka AbeInstitute of Crop Science, NARO, Tsukuba, Japan. fabe@affrc.go.jp.
Yoko KamiyaKihara Institute for Biological Research, Yokohama City University, Yokohama, Japan.
Yuji IshidaPlant Innovation Center, Japan Tobacco Inc. (currently Agri-Bio Research Center, KANEKA CORPORATION), Iwata, Japan.
Hiroshi HisanoInstitute of Plant Science & Resources, Okayama University, Kurashiki, Japan.
Kanako KawauraKihara Institute for Biological Research, Yokohama City University, Yokohama, Japan.
Toshihiko KomariPlant Innovation Center, Japan Tobacco Inc. (currently Agri-Bio Research Center, KANEKA CORPORATION), Iwata, Japan.
Kazuhiro SatoInstitute of Plant Science & Resources, Okayama University, Kurashiki, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Knockout mutants provide definitive information about the functions of genes related to agronomic traits, including seed dormancy. However, it takes many years to produce knockout mutants using conventional techniques in polyploid plants such as hexaploid wheat. Genome editing with sequence-specific nucleases is a promising approach for obtaining knockout mutations in all targeted homoeologs of wheat simultaneously. Here, we describe a procedure to produce a triple recessive mutant in wheat via genome editing. This protocol covers the evaluation of gRNA and Agrobacterium-mediated transformation to obtain edited wheat seedlings.

Indexed as

CRISPR-Cas SystemsGene EditingGene Knockout TechniquesPlant DormancyTriticumAgrobacteriumGenes, PlantGenome, PlantMutationPlants, Genetically ModifiedRNA, Guide, CRISPR-Cas SystemsSeedlingsSeedsRNA, Guide, CRISPR-Cas SystemsAgrobacterium-mediated transformationCRISPR/Cas9Genome editingImmature embryoWheat

Identifiers

PMID38977575

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.