Evidence map›Paper›PMID 38977331›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2024

[2, 6-dimethoxy-1, 4-benzoquinone alleviates septic shock in mice by inhibiting NLRP3 inflammasome activation].

W Zhang, M Deng, Y Zeng, C Liu, F Shang, W Xu, H Jiang, F Wang, Y Yang

Abstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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0cells of the map it votes in
2citing papers in PubMed
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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

W ZhangClinical Laboratory, First Affiliated Hospital of Bengbu Medical University, Bengbu 233004, China.
M DengClinical Laboratory, First Affiliated Hospital of Bengbu Medical University, Bengbu 233004, China.
Y ZengClinical Laboratory, First Affiliated Hospital of Bengbu Medical University, Bengbu 233004, China.
C LiuClinical Laboratory, First Affiliated Hospital of Bengbu Medical University, Bengbu 233004, China.
F ShangAnhui Provincial Key Laboratory of Immunology in Chronic Diseases, Bengbu Medical University, Bengbu 233030, China.
W XuClinical Laboratory, First Affiliated Hospital of Bengbu Medical University, Bengbu 233004, China.
H JiangAnhui Provincial Key Laboratory of Immunology in Chronic Diseases, Bengbu Medical University, Bengbu 233030, China.
F WangClinical Laboratory, First Affiliated Hospital of Bengbu Medical University, Bengbu 233004, China.
Y YangClinical Laboratory, First Affiliated Hospital of Bengbu Medical University, Bengbu 233004, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the mechanism of 2, 6-dimethoxy-1, 4-benzoquinone (DMQ), an active ingredients in fermented wheat germ extract, for inhibiting NLRP3 inflammasome activation and alleviating septic shock in mice.

methodsCultured murine bone marrow-derived macrophages (BMDM) stimulated with lipopolysaccharide (LPS) were treated with DMQ, followed by treatment with Nigericin, ATP, and MSU for activating the canonical NLRP3 inflammasome; the noncanonical NLRP3 inflammasome was activated by intracellular transfection of LPS, and AIM2 inflammasome was activated using Poly A: T.In human monocytic THP-1 cells, the effect of Nigericin on inflammasome activation products was examined using Western blotting and ELISA.Co-immunoprecipitation was performed to explore the mechanism of DMQ-induced blocking of NLRP3 inflammasome activation.In a male C57BL/6J mouse model of LPS-induced septic shock treated with 20 and 40 mg/kg DMQ, the levels of IL-1β and TNF-α in the serum and peritoneal lavage fluid were determined using ELISA, and the survival time of the mice within 36 h was observed.

resultsTreatment with DMQ effectively inhibited LPS-induced activation of canonical NLRP3 inflammasome in mouse BMDM and human THP-1 cells and also inhibited non-canonical NLRP3 inflammasome activation in mouse BMDM, but produced no significant effect on AIM2 inflammasome activation.DMQ significantly blocked the binding between ASC and NLRP3.In the mouse models of septic shock, DMQ treatment significantly reduced the levels of IL-1β in the serum and peritoneal fluid and obviously prolonged survival time of the mice.

conclusionDMQ can effectively block ASC-NLRP3 interaction to inhibit NLRP3 inflammasome activation and alleviate LPSinduced septic shock in mice.

Indexed as

BenzoquinonesInflammasomesInterleukin-1betaLipopolysaccharidesMice, Inbred C57BLNLR Family, Pyrin Domain-Containing 3 ProteinShock, SepticAnimalsDisease Models, AnimalHumansMacrophagesMaleMiceTHP-1 CellsTumor Necrosis Factor-alphaBenzoquinonesInflammasomesInterleukin-1betaLipopolysaccharidesNLR Family, Pyrin Domain-Containing 3 ProteinNlrp3 protein, mouseTumor Necrosis Factor-alpha2, 6-dimethoxy-1, 4-benzoquinonefermented wheat germ extractNLRP3 inflammasomeseptic shock

Identifiers

PMID38977331
PMCPMC11237302

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.