Evidence map›Paper›PMID 38975762›Full record

ArticleApplied and environmental microbiology2024

Efficient pathway-driven

Ryota Kurashiki, Masahiro Takahashi, Yuta Okumura, Tatsuya Ono, Hirofumi Endo, Kohei Makino, Kaho Fukui, Kyosuke Yokoyama, Shu Ishikawa, Ken-Ichi Yoshida and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied and environmental microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Ryota KurashikiDepartment of Engineering, Graduate School of Sustainability Science, Tottori University, Tottori, Japan.
Masahiro TakahashiIMRA Japan Co., Ltd., Kariya, Japan.
Yuta OkumuraDepartment of Engineering, Graduate School of Sustainability Science, Tottori University, Tottori, Japan.
Tatsuya OnoDepartment of Engineering, Graduate School of Sustainability Science, Tottori University, Tottori, Japan.
Hirofumi EndoDepartment of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori, Japan.
Kohei MakinoGraduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan.
Kaho FukuiGraduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan.
Kyosuke YokoyamaGraduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan.
Shu IshikawaGraduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan.
Ken-Ichi YoshidaGraduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan.ORCID 0000-0002-3383-4664
Takashi OhshiroFaculty of Engineering, Tottori University, Tottori, Japan.
Hirokazu SuzukiFaculty of Engineering, Tottori University, Tottori, Japan.ORCID 0000-0002-9507-6467

Funding

Noda Institute for Scientific Research (NISR)
6 · The paper itself

Abstract

Mesophilic enzymes, which are active at moderate temperatures, may dominate enzymatic reactions even in the presence of thermophilic crude enzymes. This study was conducted to investigate this hypothesis with mesophilic inositol dehydrogenases (IolG and IolX) produced in IMPORTANCE: Enzyme cocktails are commonly employed for cell-free chemical synthesis; however, their preparation involves cumbersome processes. This study affirms that mesophilic enzymes in thermophilic crude extracts can function as specific catalysts at moderate temperatures, akin to enzyme cocktails. The catalyst was prepared by permeabilizing cells without the need for concentration, extraction, or purification processes; hence, its preparation was considerably simpler compared with conventional methods for enzyme cocktails. This approach was employed to produce pure

Indexed as

Bacterial ProteinsGeobacillusInositolBacillus subtilisEscherichia coliPromoter Regions, GeneticSugar Alcohol DehydrogenasesBacterial ProteinsInositolscyllitolSugar Alcohol DehydrogenasesBacillusbioconversioncell-free synthesisenzyme cocktailGeobacillusiol genepathway engineering

Identifiers

PMID38975762
PMCPMC11267878

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.