ArticleaBIOTECH2024
Optimized protoplast isolation and transfection with a breakpoint: accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot.
Article in aBIOTECH, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- AI-designed OpenCRISPR-1 performs robust knockout, base editing, and prime editing in rice.The New phytologist · 2026Article
- Genetic transformation and CRISPR/Cas12a-mediated gene editing of European beech (Fagus sylvatica L.) employing a transient protoplast system.Communications biology · 2026Article
- Seeds and nanomaterials: seed-assisted synthesis, nanotoxicity, and seed-associated nanotechnology innovations.Journal of experimental botany · 2026Review
- Mapping cis-regulatory mutations at scale in sorghum enables modulation of gene expression.Nature biotechnology · 2026Article
- High levels of ADAR overexpression induce abundant and stochastic off-target RNA editing in rice protoplasts.aBIOTECH · 2026Article
- A Stable and Rapid Protoplast-Based CRISPR/Cas9-Ribonucleoprotein Complex Screening System for Octoploid Strawberry (Plants (Basel, Switzerland) · 2026Article
- Protoplast isolation and transient gene expression in Suaeda aralocaspica, a halophyte with single-cell CPlant methods · 2026Article
- Establishing Reagent Testing Platforms for Functional Analyses in Sunflower.Plants (Basel, Switzerland) · 2025Article
- Effect of Suberoylanilide Hydroxamic Acid and Phytosulfokine-Alpha on Successful Plant Regeneration from Embryogenic Callus-Derived Protoplasts of Garlic (International journal of molecular sciences · 2025Article
- Multi-omics reveals DEAD-box RNA helicase 20 as key protein enhancing wilt resistance in transgenic chickpea.Plant cell reports · 2025Article
- A novel protocol for protoplast isolation, transfection, and culture in Cannabis sativa L.BMC plant biology · 2025Article
- PVP-40 mediated enhancement of mesophyll protoplast yield and viability for transient gene expression in black huckleberry.Plant methods · 2025Article
- Establishing a reliable protoplast system for grapevine: isolation, transformation, and callus induction.Protoplasma · 2025Article
- Stable transgene expression and CRISPR-mediated knock-in system of a bacteria-derived antibiotic selection gene in the green alga Ulva prolifera.BMC plant biology · 2025Article
- Protoplast-Based Regeneration Enables CRISPR/Cas9 Application in Two TemperatePlants (Basel, Switzerland) · 2025Article
- Comprehensive benchmarking of genome editing quantification methods for plant applications.iScience · 2025Article
- Streamlined protoplast transfection system for in-vivo validation and transgene-free genome editing in Banana.Transgenic research · 2025Article
- Long-read genome sequencing reveals the sequence characteristics of pear self-incompatibility locus.Molecular horticulture · 2025Article
- A simple and efficient system for evaluating plant genome editing efficiency and its application in optimizing the ISAam1 TnpB nuclease.Frontiers in plant science · 2025Article
- Adaptation of bacterial natural single guide RNA (tracr-L) for efficient plant genome editing.Plant cell reports · 2024Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CRISPR-Cas genome editing tools are revolutionizing agriculture and basic biology with their simplicity and precision ability to modify target genomic loci. Software-predicted guide RNAs (gRNAs) often fail to induce efficient cleavage at target loci. Many target loci are inaccessible due to complex chromatin structure. Currently, there is no suitable tool available to predict the architecture of genomic target sites and their accessibility. Hence, significant time and resources are spent on performing editing experiments with inefficient guides. Although in vitro-cleavage assay could provide a rough assessment of gRNA efficiency, it largely excludes the interference of native genomic context. Transient in-vivo testing gives a proper assessment of the cleavage ability of editing reagents in a native genomic context. Here, we developed a modified protocol that offers highly efficient protoplast isolation from rice, Supplementary Information: The online version contains supplementary material available at 10.1007/s42994-024-00139-7.
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Registered trials
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