ArticleaBIOTECH2024
Simultaneous genetic transformation and genome editing of mixed lines in soybean (
Article in aBIOTECH, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- GmRPS5 Promoter-Driven CRISPR/LbCas12a Efficiently Generates Soybean Sextuple Mutants.Plant biotechnology journal · 2026Article
- Improved efficiency of genetic transformation for the development of elite germplasm using CRISPR/Cas12i3 in maize.aBIOTECH · 2026Article
- Development of high-throughput tissue culture-free plant transformation systems.The Plant journal : for cell and molecular biology · 2025Review
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Authors and funding
10 authors.
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Abstract
Robust genome editing technologies are becoming part of the crop breeding toolbox. Currently, genome editing is usually conducted either at a single locus, or multiple loci, in a variety at one time. Massively parallel genomics platforms, multifaceted genome editing capabilities, and flexible transformation systems enable targeted variation at nearly any locus, across the spectrum of genotypes within a species. We demonstrate here the simultaneous transformation and editing of many genotypes, by targeting mixed seed embryo explants with genome editing machinery, followed by re-identification through genotyping after plant regeneration. Transformation and Editing of Mixed Lines (TREDMIL) produced transformed individuals representing 101 of 104 (97%) mixed elite genotypes in soybean; and 22 of 40 (55%) and 9 of 36 (25%) mixed maize female and male elite inbred genotypes, respectively. Characterization of edited genotypes for the regenerated individuals identified over 800 distinct edits at the Determinate1 ( Supplementary Information: The online version contains supplementary material available at 10.1007/s42994-024-00173-5.
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