Evidence map›Paper›PMID 38973210›Full record

ArticleACS sensors2024

Split Luciferase Molecular Tension Sensors for Bioluminescent Readout of Mechanical Forces in Biological Systems.

Brian L Zhong, Jeandele M Elliot, Pengli Wang, Hongquan Li, R Nelson Hall, Bo Wang, Manu Prakash, Alexander R Dunn

Abstract readLetter
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS sensors, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Luciferase complementation for cellular assays beyond protein-protein interactions.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Brian L ZhongDepartment of Chemical Engineering, Stanford University, Stanford, California 94305, United States.
Jeandele M ElliotDepartment of Chemical Engineering, Stanford University, Stanford, California 94305, United States.
Pengli WangDepartment of Chemical Engineering, Stanford University, Stanford, California 94305, United States.
Hongquan LiDepartment of Electrical Engineering, Stanford University, Stanford, California 94305, United States.
R Nelson HallDepartment of Bioengineering, Stanford University, Stanford, California 94305, United States.
Bo WangDepartment of Bioengineering, Stanford University, Stanford, California 94305, United States.
Manu PrakashDepartment of Bioengineering, Stanford University, Stanford, California 94305, United States.
Alexander R DunnDepartment of Chemical Engineering, Stanford University, Stanford, California 94305, United States.ORCID 0000-0001-6096-4600

Funding

Molecular mechanisms underlying force transduction at cellular adhesion complexesR35GM130332 · NIGMS · STANFORD UNIVERSITY · PI Alexander R Dunn · 2019 to 2026
$4.9M
Stanford Chem-H Chemistry/Biology Interface Predoctoral Training ProgramT32GM120007 · NIGMS · STANFORD UNIVERSITY · PI BERTOZZI, CAROLYN · 2016 to 2020
$1.2M
NIGMS NIH HHS R35 GM130332NIGMS NIH HHS T32 GM120007
6 · The paper itself

Abstract

The ability of proteins to sense and transmit mechanical forces underlies many biological processes, but characterizing these forces in biological systems remains a challenge. Existing genetically encoded force sensors typically rely on fluorescence or bioluminescence resonance energy transfer (FRET or BRET) to visualize tension. However, these force sensing modules are relatively large, and interpreting measurements requires specialized image analysis and careful control experiments. Here, we report a compact molecular tension sensor that generates a bioluminescent signal in response to tension. This sensor (termed PILATeS) makes use of the split NanoLuc luciferase and consists of the

Indexed as

Biosensing TechniquesLuciferasesLuminescent MeasurementsConnectinHumansVinculinConnectinLuciferasesTTN protein, humanVinculinbioluminescencefocal adhesionforce sensorluciferasemechanobiologyprotein engineering

Identifiers

PMID38973210
PMCPMC11839233

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.