Evidence map›Paper›PMID 38969982›Full record

ArticleBiological procedures online2024

EAF2 Downregulation Recruits Tumor-associated Macrophages in Prostate Cancer through Upregulation of MIF.

Tianyu Cao, Qian Sun, Xiaoqin Shi, Xiuke Lin, Qingyuan Lin, Jinchao Zhu, Junhao Xu, Di Cui, Youwei Shi, Yifeng Jing and 1 more

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In one paragraph

Article in Biological procedures online, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Tianyu Cao *Department of Pathology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Qian Sun *Department of Urology, Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Xiaoqin Shi *Department of Pathology , Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Xiuke Lin *Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen, China.
Qingyuan LinDepartment of Pathology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Jinchao ZhuDepartment of Pathology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Junhao XuDepartment of Urology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Di CuiDepartment of Urology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China. cuidi@sjtu.edu.cn.
Youwei ShiDepartment of Urology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China. shiyouwei@alumni.sjtu.edu.cn.
Yifeng JingDepartment of Urology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China. jingyifeng@sjtu.edu.cn.
Wenhuan GuoDepartment of Pathology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China. wenhuan1130@sina.com.

Funding

National Natural Science Foundation of China 81502212
6 · The paper itself

Abstract

backgroundThe role of tumor inflammatory microenvironment in the advancement of cancer, particularly prostate cancer, is widely acknowledged. ELL-associated factor 2 (EAF2), a tumor suppressor that has been identified in the prostate, is often downregulated in prostate cancer. Earlier investigations have shown that mice with EAF2 gene knockout exhibited a substantial infiltration of inflammatory cells into the prostatic stroma.

methodsA cohort comprising 38 patients who had been diagnosed with prostate cancer and subsequently undergone radical prostatectomy (RP) was selected. These patients were pathologically graded according to the Gleason scoring system and divided into two groups. The purpose of this selection was to investigate the potential correlation between EAF2 and CD163 using immunohistochemistry (IHC) staining. Additionally, in vitro experimentation was conducted to verify the relationship between EAF2 expression, macrophage migration and polarization.

resultsOur study demonstrated that in specimens of human prostate cancer, the expression of EAF2 was notably downregulated, and this decrease was inversely associated with the number of CD163-positive macrophages that infiltrated the cancerous tissue. Cell co-culture experiments revealed that the chemotactic effect of tumor cells towards macrophages was intensified and that macrophages differentiated into tumor-associated macrophages (TAMs) when EAF2 was knocked out. Additionally, the application of cytokine protein microarray showed that the expression of chemokine macrophage migration inhibitory factor (MIF) increased after EAF2 knockout.

conclusionsOur findings suggested that EAF2 was involved in the infiltration of CD163-positive macrophages in prostate cancer via MIF.

Indexed as

EAF2MIFProstate cancerTumor Associated Macrophage

Identifiers

PMID38969982
PMCPMC11225222

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