Evidence map›Paper›PMID 38969067›Full record

ArticleMucosal immunology2024

Viral-vectored boosting of OmcB- or CPAF-specific T-cell responses fail to enhance protection from Chlamydia muridarum in infection-immune mice and elicits a non-protective CD8-dominant response in naïve mice.

Taylor B Poston, Jenna Girardi, A Grace Polson, Aakash Bhardwaj, Kacy S Yount, Ian Jaras Salas, Logan K Trim, Yanli Li, Catherine M O'Connell, Darren Leahy and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Mucosal immunology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. CD8iScience · 2026
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Taylor B PostonDepartment of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. Electronic address: taylor_poston@med.unc.edu.
Jenna GirardiDepartment of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
A Grace PolsonDepartment of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Aakash BhardwajDepartment of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Kacy S YountDepartment of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Ian Jaras SalasDepartment of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Logan K TrimCenter for Immunology and Infection Control and School of Biomedical Sciences, Queensland University of Technology, Brisbane, Queensland, Australia.
Yanli LiDepartment of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Catherine M O'ConnellDepartment of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Darren LeahyCenter for Immunology and Infection Control and School of Biomedical Sciences, Queensland University of Technology, Brisbane, Queensland, Australia.
Jonathan M HarrisCenter for Immunology and Infection Control and School of Biomedical Sciences, Queensland University of Technology, Brisbane, Queensland, Australia.
Kenneth W BeagleyCenter for Immunology and Infection Control and School of Biomedical Sciences, Queensland University of Technology, Brisbane, Queensland, Australia.
Nilu GoonetillekeDepartment of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Toni DarvilleDepartment of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Funding

University of North Carolina - Chlamydia Vaccine Initiative (UNC-CVI)U19AI144181 · NIAID · UNIV OF NORTH CAROLINA CHAPEL HILL · PI DARVILLE, TONI · 2019 to 2023
$10.7M
NIAID NIH HHS U19 AI144181
6 · The paper itself

Abstract

A vaccine is needed to combat the Chlamydia epidemic. Replication-deficient viral vectors are safe and induce antigen-specific T-cell memory. We tested the ability of intramuscular immunization with modified vaccinia Ankara (MVA) virus or chimpanzee adenovirus (ChAd) expressing chlamydial outer membrane protein (OmcB) or the secreted protein, chlamydial protease-like activating factor (CPAF), to enhance T-cell immunity and protection in mice previously infected with plasmid-deficient Chlamydia muridarum CM972 and elicit protection in naïve mice. MVA.OmcB or MVA.CPAF increased antigen-specific T cells in CM972-immune mice ∼150 and 50-fold, respectively, but failed to improve bacterial clearance. ChAd.OmcB/MVA.OmcB prime-boost immunization of naïve mice elicited a cluster of differentiation (CD) 8-dominant T-cell response dominated by cluster of differentiation (CD)8 T cells that failed to protect. ChAd.CPAF/ChAd.CPAF prime-boost also induced a CD8-dominant response with a marginal reduction in burden. Challenge of ChAd.CPAF-immunized mice genetically deficient in CD4 or CD8 T cells showed that protection was entirely CD4-dependent. CD4-deficient mice had prolonged infection, whereas CD8-deficient mice had higher frequencies of CPAF-specific CD4 T cells, earlier clearance, and reduced burden than wild-type controls. These data reinforce the essential nature of the CD4 T-cell response in protection from chlamydial genital infection in mice and the need for vaccine platforms that drive CD4-dominant responses.

Indexed as

Bacterial Outer Membrane ProteinsBacterial VaccinesCD8-Positive T-LymphocytesChlamydia InfectionsChlamydia muridarumGenetic VectorsImmunization, SecondaryAdenoviridaeAnimalsEndopeptidasesFemaleHumansMiceMice, Inbred C57BLVaccinia virusBacterial Outer Membrane ProteinsBacterial VaccinesCPA factorEndopeptidases

Identifiers

PMID38969067
PMCPMC11495396

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.