Evidence map›Paper›PMID 38968989›Full record

ArticleDevelopmental biology2024

Reversion induced LIM domain protein (RIL) is a Daam1-interacting protein and regulator of the actin cytoskeleton during non-canonical Wnt signaling.

Courtney Mezzacappa, Yuko Komiya, Raymond Habas

Abstract read
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In one paragraph

Article in Developmental biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. SNP-Based and Kmer-Based eQTL Analysis Using Transcriptome Data.Animals : an open access journal from MDPI · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Courtney MezzacappaDepartment of Biology, Temple University, Philadelphia, PA, 19122, USA.
Yuko KomiyaDepartment of Biology, Temple University, Philadelphia, PA, 19122, USA.
Raymond HabasDepartment of Biology, Temple University, Philadelphia, PA, 19122, USA. Electronic address: habas@temple.edu.

Funding

Non-canonical Wnt signaling and cell motilityR01GM078172 · NIGMS · UNIV OF MED/DENT NJ-R W JOHNSON MED SCH · PI HABAS, RAYMOND · 2007 to 2018
$3.1M
Understanding The Role of Custos in Canonical Wnt SignalingR01GM115929 · NIGMS · TEMPLE UNIV OF THE COMMONWEALTH · PI HABAS, RAYMOND · 2015 to 2018
$1.2M
The role of SRGAP2 in vertebrate gastrulation and neural tube closureR03HD082597 · NICHD · TEMPLE UNIV OF THE COMMONWEALTH · PI HABAS, RAYMOND · 2015 to 2016
$155k
Characterization of two proteins that regulate vertebrateR03HD107523 · NICHD · TEMPLE UNIV OF THE COMMONWEALTH · PI HABAS, RAYMOND · 2022 to 2023
$155k
NICHD NIH HHS R03 HD082597NICHD NIH HHS R03 HD107523NIGMS NIH HHS R01 GM078172NIGMS NIH HHS R01 GM115929
6 · The paper itself

Abstract

The Daam1 protein regulates Wnt-induced cytoskeletal changes during vertebrate gastrulation though its full mode of action and binding partners remain unresolved. Here we identify Reversion Induced LIM domain protein (RIL) as a new interacting protein of Daam1. Interaction studies uncover binding of RIL to the C-terminal actin-nucleating portion of Daam1 in a Wnt-responsive manner. Immunofluorescence studies showed subcellular localization of RIL to actin fibers and co-localization with Daam1 at the plasma membrane. RIL gain- and loss-of-function approaches in Xenopus produced severe gastrulation defects in injected embryos. Additionally, a simultaneous loss of Daam1 and RIL synergized to produce severe gastrulation defects indicating RIL and Daam1 may function in the same signaling pathway. RIL further synergizes with another novel Daam1-interacting protein, Formin Binding Protein 1 (FNBP1), to regulate gastrulation. Our studies altogether show RIL mediates Daam1-regulated non-canonical Wnt signaling that is required for vertebrate gastrulation.

Indexed as

Actin CytoskeletonGastrulationMicrofilament ProteinsWnt Signaling PathwayXenopus laevisXenopus ProteinsActinsAdaptor Proteins, Signal TransducingAnimalsDNA-Binding ProteinsFemaleHumansLIM Domain ProteinsProtein BindingRatsrho GTP-Binding ProteinsActinsAdaptor Proteins, Signal TransducingDAAM1 protein, humanDaam1 protein, XenopusDNA-Binding ProteinsLIM Domain ProteinsMicrofilament ProteinsPdlim4 protein, ratrho GTP-Binding ProteinsXenopus Proteins

Identifiers

PMID38968989
PMCPMC11321505

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.