Evidence map›Paper›PMID 38968349›Full record

ArticleScience advances2024

Piggybacking functionalized DNA nanostructures into live-cell nuclei.

Golbarg M Roozbahani, P L Colosi, Attila Oravecz, Elena M Sorokina, Wolfgang Pfeifer, Siamak Shokri, Yin Wei, Pascal Didier, Marcello DeLuca, Gaurav Arya and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. Article
  3. Molecular tags for electron cryo-tomography.Emerging topics in life sciences · 2025
    Review
  4. Review
  5. Article
  6. Article
  7. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Golbarg M RoozbahaniDepartment of Physics, The Ohio State University, Columbus, OH 43210, USA.ORCID 0000-0002-4076-9020
P L ColosiDepartment of Physiology, University of Pennsylvania, Philadelphia, PA 19104, USA.ORCID 0000-0001-6802-4998
Attila OraveczInstitut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch 67404, France.ORCID 0000-0002-7860-6606
Elena M SorokinaDepartment of Physiology, University of Pennsylvania, Philadelphia, PA 19104, USA.ORCID 0000-0003-2079-869X
Wolfgang PfeiferDepartment of Physics, The Ohio State University, Columbus, OH 43210, USA.ORCID 0000-0002-5589-8415
Siamak ShokriDepartment of Physics, The Ohio State University, Columbus, OH 43210, USA.ORCID 0009-0000-8306-0620
Yin WeiBiophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA.ORCID 0000-0001-9915-5317
Pascal DidierUniversité de Strasbourg, Illkirch 67404, France.ORCID 0000-0003-2850-9186
Marcello DeLucaDepartment of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708, USA.ORCID 0000-0002-4299-3501
Gaurav AryaDepartment of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708, USA.ORCID 0000-0002-5615-0521
László ToraInstitut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch 67404, France.ORCID 0000-0001-7398-2250
Melike LakadamyaliDepartment of Physiology, University of Pennsylvania, Philadelphia, PA 19104, USA.ORCID 0000-0002-7524-6414
Michael G PoirierDepartment of Physics, The Ohio State University, Columbus, OH 43210, USA.ORCID 0000-0002-1563-5792
Carlos E CastroDepartment of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.ORCID 0000-0001-7023-6105

Funding

Mechanisms of chromatin regulation of transcriptionR35GM139564 · NIGMS · OHIO STATE UNIVERSITY · PI POIRIER, MICHAEL GUY · 2021 to 2025
$3.6M
NIGMS NIH HHS R35 GM139564
6 · The paper itself

Abstract

DNA origami nanostructures (DOs) are promising tools for applications including drug delivery, biosensing, detecting biomolecules, and probing chromatin substructures. Targeting these nanodevices to mammalian cell nuclei could provide impactful approaches for probing, visualizing, and controlling biomolecular processes within live cells. We present an approach to deliver DOs into live-cell nuclei. We show that these DOs do not undergo detectable structural degradation in cell culture media or cell extracts for 24 hours. To deliver DOs into the nuclei of human U2OS cells, we conjugated 30-nanometer DO nanorods with an antibody raised against a nuclear factor, specifically the largest subunit of RNA polymerase II (Pol II). We find that DOs remain structurally intact in cells for 24 hours, including inside the nucleus. We demonstrate that electroporated anti-Pol II antibody-conjugated DOs are piggybacked into nuclei and exhibit subdiffusive motion inside the nucleus. Our results establish interfacing DOs with a nuclear factor as an effective method to deliver nanodevices into live-cell nuclei.

Indexed as

Cell NucleusDNANanostructuresCell Line, TumorHumansNanotubesRNA Polymerase IIDNARNA Polymerase II

Identifiers

PMID38968349
PMCPMC11225781

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.