Evidence map›Paper›PMID 38967463›Full record

ArticleMicrobiology resource announcements2024

Mobile-CRISPRi protocol optimization for

Logan J Geyman, Madeline P Tanner, Natalia Rosario-Meléndez, Jason M Peters, Mark J Mandel, Julia C van Kessel

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology resource announcements, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Logan J GeymanDepartment of Biology, Indiana University , Bloomington, Indiana, USA.ORCID 0000-0001-6697-6725
Madeline P TannerDepartment of Biology, Indiana University , Bloomington, Indiana, USA.
Natalia Rosario-MeléndezDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison , Madison, Wisconsin, USA.ORCID 0000-0003-1665-4167
Jason M PetersMicrobiology Doctoral Training Program, University of Wisconsin-Madison , Madison, Wisconsin, USA.
Mark J MandelDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison , Madison, Wisconsin, USA.ORCID 0000-0001-6506-6711
Julia C van KesselDepartment of Biology, Indiana University , Bloomington, Indiana, USA.ORCID 0000-0002-1612-2403

Funding

Biotechnology Training ProgramT32GM135066 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI SCOTT M. COYLE, BRIAN G FOX · 2020 to 2026
$7.0M
Quorum sensing regulation of bacterial developmentR35GM124698 · NIGMS · TRUSTEES OF INDIANA UNIVERSITY · PI Julia C. van Kessel · 2017 to 2026
$4.1M
Genetic analysis of beneficial bacterial colonizationR35GM148385 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Mark J Mandel · 2023 to 2026
$1.8M
Cross-species Analysis of Bacterial Gene NetworksR35GM150487 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Jason M. Peters · 2023 to 2026
$1.5M
HHS | National Institutes of Health (NIH) R35GM124698HHS | National Institutes of Health (NIH) R35GM148385HHS | National Institutes of Health (NIH) R35GM150487HHS | National Institutes of Health (NIH) T32GM135066NIGMS NIH HHS R35 GM124698NIGMS NIH HHS R35 GM148385NIGMS NIH HHS R35 GM150487NIGMS NIH HHS T32 GM135066NSF | National Science Foundation Graduate Research Fellowship Program (GRFP)
6 · The paper itself

Abstract

Mobile clustered regularly interspaced palindromic repeats interference (Mobile-CRISPRi) is an established method for bacterial gene expression knockdown. The deactivated Cas9 protein and guide RNA are isopropyl β-D-1-thiogalactopyranoside inducible, and all components are integrated into the chromosome via Tn

Indexed as

CRISPRCRISPRiregulation of gene expressionVibrioVibrio campbelliiVibrio choleraeVibrio fischeriVibrio parahaemolyticusVibrio vulnificus

Identifiers

PMID38967463
PMCPMC11320974

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.