Evidence map›Paper›PMID 38963646›Full record

ArticleBiological trace element research2025

Evaluation of Boric Acid Treatment on microRNA-127-5p and Metastasis Genes Orchestration of Breast Cancer Stem Cells.

Tuğba Semerci Sevimli, Aynaz Ghorbani, Fidan Gakhiyeva, Aliakbar Ebrahimi, Hamed Ghorbanpoor, Burcugül Altuğ, Fulya Buge Ergen, Zarifa Ahmadova, Merve Nur Soykan, Emre Tufekcioglu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biological trace element research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Tuğba Semerci SevimliCellular Therapy and Stem Cell Production, Application, and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, 26040, Turkey. tssevimli@ogu.edu.tr.
Aynaz GhorbaniCellular Therapy and Stem Cell Production, Application, and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, 26040, Turkey.ORCID http://orcid.org/0000-0001-5516-027X
Fidan GakhiyevaCellular Therapy and Stem Cell Production, Application, and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, 26040, Turkey.ORCID http://orcid.org/0000-0002-0124-4694
Aliakbar EbrahimiCellular Therapy and Stem Cell Production, Application, and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, 26040, Turkey.ORCID http://orcid.org/0000-0001-6437-7796
Hamed GhorbanpoorCellular Therapy and Stem Cell Production, Application, and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, 26040, Turkey.ORCID http://orcid.org/0000-0002-2665-8172
Burcugül AltuğCellular Therapy and Stem Cell Production, Application, and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, 26040, Turkey.ORCID http://orcid.org/0000-0003-4460-8467
Fulya Buge ErgenCellular Therapy and Stem Cell Production, Application, and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, 26040, Turkey.ORCID http://orcid.org/0000-0001-8089-9033
Zarifa AhmadovaDepartment of Surgery, Medical Faculty Mannheim, Heidelberg University, 68167, Mannheim, Germany.ORCID http://orcid.org/0000-0003-0883-2752
Merve Nur SoykanCellular Therapy and Stem Cell Production, Application, and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, 26040, Turkey.ORCID http://orcid.org/0000-0003-1231-9791
Emre TufekciogluDepartment of Industrial Design, Faculty of Architecture and Design, Eskisehir Technical University, Eskisehir, 26555, Turkey.ORCID http://orcid.org/0000-0002-5304-7633

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Coregulation of microRNAs (miRNAs) and cancer stem cells (CSCs) is very important in carcinogenesis. miR-127-5p is known to be downregulated in breast cancer. In this study, we aimed to investigate how boric acid (BA), known for its previously unstudied anti-cancer properties, would affect the expression of miR127-5p and genes responsible for breast cancer stem cells (BC-SCs) metastasis. BC-SCs were isolated from human breast cancer cells (MCF-7) by immunomagnetic cell separation and characterized with flow cytometry and sphere formation. The viability of BC-SCs and the determination of its IC50 value in response to boric acid (BA) were assessed via the MTT assay. Boric acid exhibited dose- and time-dependent inhibition of cell viability in cells. The IC50 doses of boric acid in MCF-7 cells and BC-SCs were 45.69 mM and 41.27 mM, respectively. The impact of BA on the expression of metastatic genes and miR127-5p was elucidated through RT-qPCR analysis. While the expression of the COL1A1 (p < 0.05) and VIM (p < 0.01) was downregulated, the expression of the miR-127-5p, ZEB1 (p < 0.01), CDH1 (p < 0.05), ITGB1 (p < 0.05), ITGA5 (p < 0.05), LAMA5 (p < 0.01), and SNAIL (p < 0.05), was up-regulated in dose-treated BC-SCs (p < 0.001) to the RT-qPCR results. Our findings suggest that boric acid could induce miR-127-5p expression. However, it cannot be said that it improves the metastasis properties of breast cancer stem cells.

Indexed as

Boric AcidsBreast NeoplasmsMicroRNAsNeoplastic Stem CellsCell SurvivalFemaleGene Expression Regulation, NeoplasticHumansMCF-7 CellsNeoplasm Metastasisboric acidBoric AcidsMicroRNAsBoric acidBreast cancerCancer stem cellMetastasismiR-127-5p

Identifiers

PMID38963646
PMCPMC11872770

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.