Study of metabolite differences of flue-cured tobacco from Canada (CT157) and Yunnan (Yunyan 87).

In order to comprehend the dissimilarities in tobacco quality between Canada and Yunnan, a comparison of the aroma components was conducted using GC-MS and HPLC analysis, coupled with orthogonal partial least squares discriminant analysis (OPLS-DA). The study revealed the detection of a total of 81 aroma components and 22 non-volatile components in both varieties of tobacco leaves. Specifically, there were 102 components of Canada tobacco leaves and 103 components of Yunnan tobacco leaves. Subsequently, a screening was performed on these two types of tobacco leaves, identifying 51 differential components, which accounted for approximately 49.5 % of the overall components detected. Among these, Canada tobacco exhibited a higher concentration of 22 components, comprising roughly 36.4 % of the total, which were primarily composed of semi-volatile organic acids and sesquiterpenes. On the other hand, Yunnan tobacco was characterized by a comparatively higher content of 43 components, constituting approximately 63.6 %, including fatty acid esters, phenols, diterpenes, sugars, and amino acids. Comparatively, Canada tobacco demonstrated elevated levels of fatty acids and sesquiterpenes, while the content of fatty acid esters and diterpenes was relatively lower. These distinctions in aroma components potentially contribute to the varied sensory aroma profiles exhibited by the two types of tobacco.