Evidence map›Paper›PMID 38961189›Full record

ArticleScientific reports2024

NF-κB inhibitor alpha controls SARS-CoV-2 infection in ACE2-overexpressing human airway organoids.

Camille R Simoneau, Pei-Yi Chen, Galen K Xing, Jennifer M Hayashi, Irene P Chen, Mir M Khalid, Nathan L Meyers, Taha Y Taha, Kristoffer E Leon, Rahul K Suryawanshi and 16 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Unique nasal cell states induced by common pediatric respiratory viruses.bioRxiv : the preprint server for biology · 2026
    Article
  5. Review
  6. Article
  7. Review
  8. Article
  9. Article
  10. The Mac1 ADP-ribosylhydrolase is a Therapeutic Target for SARS-CoV-2.bioRxiv : the preprint server for biology · 2025
    Article
  11. Review
  12. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

26 authors.

Camille R Simoneau *Gladstone Institute of Virology, San Francisco, CA, USA.
Pei-Yi Chen *Gladstone Institute of Virology, San Francisco, CA, USA.
Galen K Xing *Chan-Zuckerberg Biohub, San Francisco, CA, USA.
Jennifer M HayashiGladstone Institute of Virology, San Francisco, CA, USA.
Irene P ChenGladstone Institute of Virology, San Francisco, CA, USA.
Mir M KhalidGladstone Institute of Virology, San Francisco, CA, USA.
Nathan L MeyersGladstone Institute of Virology, San Francisco, CA, USA.
Taha Y TahaGladstone Institute of Virology, San Francisco, CA, USA.
Kristoffer E LeonGladstone Institute of Virology, San Francisco, CA, USA.
Rahul K SuryawanshiGladstone Institute of Virology, San Francisco, CA, USA.
Maria McCavitt-MalvidoGladstone Institute of Virology, San Francisco, CA, USA.
Tal AshuachCenter for Computational Biology, University of California, Berkeley, Berkeley, CA, USA.
Krystal A FontaineGladstone Institute of Virology, San Francisco, CA, USA.
Lauren RodriguezImmunoX CoLabs, University of California San Francisco, San Francisco, CA, USA.
Bastian JoehnkDepartment of Microbiology and Immunology, University of California San Francisco, San Francisco, CA, USA.
Keith WalcottDepartment of Microbiology and Immunology, University of California San Francisco, San Francisco, CA, USA.
Sreelakshmi VasudevanMedical Service, San Francisco VA Healthcare System, San Francisco, CA, USA.
Xiaohui FangDepartment of Medicine and Department of Anesthesia, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA.
Mazharul MaishanDepartment of Medicine and Department of Anesthesia, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA.
Shawn SchultzCenter for Computational Biology, University of California, Berkeley, Berkeley, CA, USA.
Jeroen P RooseDepartment of Anatomy, University of California San Francisco, San Francisco, CA, USA.
Michael A MatthayDepartment of Medicine and Department of Anesthesia, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA.
Anita SilDepartment of Microbiology and Immunology, University of California San Francisco, San Francisco, CA, USA.
Mehrdad ArjomandiMedical Service, San Francisco VA Healthcare System, San Francisco, CA, USA.
Nir YosefCenter for Computational Biology, University of California, Berkeley, Berkeley, CA, USA. nir.yosef@weizmann.ac.il.
Melanie OttGladstone Institute of Virology, San Francisco, CA, USA. melanie.ott@gladstone.ucsf.edu.

Funding

Understand the metabolic fitness of naïve T cellsP01AI091580 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI JEROEN ROOSE · 2011 to 2026
$31.1M
RESEARCH PROJECT 2U19AI135990 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Nevan J Krogan · 2018 to 2026
$21.4M
California Tobacco-related Disease Research Program 28FT-0020California Tobacco-related Disease Research Program T29IR0715Departments of Defense W81XWH-20-1-0158NIAID NIH HHS P01 AI091580NIAID NIH HHS U19 AI135990NIH HHS 3P01AI091580-09S1NIH/NIAID 2U19AI135990NIH/NIAID P01-AI091580Veterans Affairs CXV-00125
6 · The paper itself

Abstract

As SARS-CoV-2 continues to spread worldwide, tractable primary airway cell models that recapitulate the cell-intrinsic response to arising viral variants are needed. Here we describe an adult stem cell-derived human airway organoid model overexpressing the ACE2 receptor (ACE2-OE) that supports robust viral replication while maintaining 3D architecture and cellular diversity of the airway epithelium. ACE2-OE organoids were infected with SARS-CoV-2 variants and subjected to single-cell RNA-sequencing. Interferon-lambda was upregulated in cells with low-level infection while the NF-kB inhibitor alpha gene (encoding IkBa) was consistently upregulated in infected cells, and its expression positively correlated with infection levels. Confocal microscopy showed more IkBa expression in infected than bystander cells, but found concurrent nuclear translocation of NF-kB that IkBa usually prevents. Overexpressing a nondegradable IkBa mutant reduced NF-kB translocation and increased viral infection. These data demonstrate the functionality of ACE2-OE organoids in SARS-CoV-2 research and underscore that the strength of the NF-kB feedback loop in infected cells controls viral replication.

Indexed as

Angiotensin-Converting Enzyme 2COVID-19NF-KappaB Inhibitor alphaOrganoidsSARS-CoV-2Virus ReplicationHumansNF-kappa BACE2 protein, humanAngiotensin-Converting Enzyme 2NF-kappa BNF-KappaB Inhibitor alphaNFKBIA protein, human

Identifiers

PMID38961189
PMCPMC11222426

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.