Evidence map›Paper›PMID 38961188›Full record

ArticleScientific reports2024

Dexamethasone treatment influences tendon healing through altered resolution and a direct effect on tendon cells.

Franciele Dietrich-Zagonel, Md Abdul Alim, Leo Bon Beckman, Pernilla Eliasson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Franciele Dietrich-ZagonelDepartment of Biomedical and Clinical Sciences, Faculty of Medicine and Health Science, Linköping University, 581 83, Linköping, Sweden.
Md Abdul AlimDepartment of Biomedical and Clinical Sciences, Faculty of Medicine and Health Science, Linköping University, 581 83, Linköping, Sweden.
Leo Bon BeckmanDepartment of Biomedical and Clinical Sciences, Faculty of Medicine and Health Science, Linköping University, 581 83, Linköping, Sweden.
Pernilla EliassonDepartment of Biomedical and Clinical Sciences, Faculty of Medicine and Health Science, Linköping University, 581 83, Linköping, Sweden. pernilla.eliasson@vgregion.se.ORCID 0000-0001-6718-034X

Funding

Åke Wiberg Stiftelse M20-0021Centrum för idrottsforskning 2022-0094Magnus Bergvalls Stiftelse 2019-03211Stiftelsen Lars Hiertas Minne FO2020-0178Vetenskapsrådet VR2017-00990
6 · The paper itself

Abstract

Inflammation, corticosteroids, and loading all affect tendon healing, with an interaction between them. However, underlying mechanisms behind the effect of corticosteroids and the interaction with loading remain unclear. The aim of this study was to investigate the role of dexamethasone during tendon healing, including specific effects on tendon cells. Rats (n = 36) were randomized to heavy loading or mild loading, the Achilles tendon was transected, and animals were treated with dexamethasone or saline. Gene and protein analyses of the healing tendon were performed for extracellular matrix-, inflammation-, and tendon cell markers. We further tested specific effects of dexamethasone on tendon cells in vitro. Dexamethasone increased mRNA levels of S100A4 and decreased levels of ACTA2/α-SMA, irrespective of load level. Heavy loading + dexamethasone reduced mRNA levels of FN1 and TenC (p < 0.05), while resolution-related genes were unaltered (p > 0.05). In contrast, mild loading + dexamethasone increased mRNA levels of resolution-related genes ANXA1, MRC1, PDPN, and PTGES (p < 0.03). Altered protein levels were confirmed in tendons with mild loading. Dexamethasone treatment in vitro prevented tendon construct formation, increased mRNA levels of S100A4 and decreased levels of SCX and collagens. Dexamethasone during tendon healing appears to act through immunomodulation by promoting resolution, but also through an effect on tendon cells.

Indexed as

Achilles TendonDexamethasoneTendon InjuriesWound HealingActinsAnimalsAnnexin A1Basic Helix-Loop-Helix ProteinsCollagenExtracellular MatrixMaleRatsRats, Sprague-DawleyRNA, MessengerS100 Calcium-Binding Protein A4TendonsActa2 protein, ratActinsAnnexin A1Basic Helix-Loop-Helix ProteinsCollagenDexamethasoneRNA, MessengerS100 Calcium-Binding Protein A4Scx protein, ratAchilles tendonCorticosteroidsInjuryIn vitroLoadingResolution

Identifiers

PMID38961188
PMCPMC11222440

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.