Evidence map›Paper›PMID 38958600›Full record

ArticleChembiochem : a European journal of chemical biology2024

Biosynthesis of Halogenated Tryptophans for Protein Engineering Using Genetic Code Expansion.

Yiming Guo, Linqi Cheng, Yu Hu, Mengxi Zhang, Rui Liu, Yixian Wang, Shiyu Jiang, Han Xiao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Phage-assisted continuous evolution of enzymes for noncanonical tyrosine biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  2. Article
  3. In Vivo Biosynthesis and Direct Incorporation of Noncanonical Amino Acids into Proteins.Chembiochem : a European journal of chemical biology · 2025
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yiming GuoDepartment of Chemistry, Rice University, 6100 Main Street, Houston, Texas, 77005, U.S.A.
Linqi ChengDepartment of Chemistry, Rice University, 6100 Main Street, Houston, Texas, 77005, U.S.A.
Yu HuDepartment of Chemistry, Rice University, 6100 Main Street, Houston, Texas, 77005, U.S.A.
Mengxi ZhangDepartment of Chemistry, Rice University, 6100 Main Street, Houston, Texas, 77005, U.S.A.
Rui LiuDepartment of Chemistry, Rice University, 6100 Main Street, Houston, Texas, 77005, U.S.A.
Yixian WangDepartment of Chemistry, Rice University, 6100 Main Street, Houston, Texas, 77005, U.S.A.
Shiyu JiangDepartment of Chemistry, Rice University, 6100 Main Street, Houston, Texas, 77005, U.S.A.
Han XiaoDepartment of Chemistry, Rice University, 6100 Main Street, Houston, Texas, 77005, U.S.A.ORCID https://orcid.org/0000-0002-4311-971X

Funding

Targeting Siglec-9/Sialoglycan Interactions to Enhance NK Functions During HIV InfectionR01AI165079 · NIAID · WISTAR INSTITUTE · PI ABDEL MOHSEN, MOHAMED, XIAO, HAN · 2021 to 2025
$3.1M
Engineering Proteins with Noncanonical Amino AcidsR35GM133706 · NIGMS · RICE UNIVERSITY · PI Han Xiao · 2019 to 2026
$2.9M
Modulation of Epigenetic Target in the Bone to Treat Breast Cancer MetastasisR01CA277838 · NCI · RICE UNIVERSITY · PI Han Xiao, Xiang Zhang · 2023 to 2026
$2.6M
Development of Bone-Targeting Antibodies for Ewing Sarcoma Using Genetic Code ExpansionR21CA255894 · NCI · RICE UNIVERSITY · PI XIAO, HAN, YUSTEIN, JASON · 2021 to 2023
$568k
Cancer Prevention Research Institute of Texas CPRIT RR170014Cancer Prevention & Research Institute of Texas (CPRIT)Hamill Innovation AwardJohn S. Dunn Foundation Collaborative Research AwardNCI NIH HHS R01 CA277838NCI NIH HHS R21 CA255894NIAID NIH HHS R01 AI165079NIGMS NIH HHS R35 GM133706NIH HHS R01-AI165079NIH HHS R01-CA277838NIH HHS R21-CA255894NIH HHS R35-GM133706Robert A. Welch Foundation C-1970Robert A. Welch Foundation HT9425-23-1-0494Robert A. Welch Foundation US Department of DefenseRobert A. Welch Foundation W81XWH-21-1-0789
6 · The paper itself

Abstract

Genetic Code Expansion technology offers significant potential in incorporating noncanonical amino acids into proteins at precise locations, allowing for the modulation of protein structures and functions. However, this technology is often limited by the need for costly and challenging-to-synthesize external noncanonical amino acid sources. In this study, we address this limitation by developing autonomous cells capable of biosynthesizing halogenated tryptophan derivatives and introducing them into proteins using Genetic Code Expansion technology. By utilizing inexpensive halide salts and different halogenases, we successfully achieve the selective biosynthesis of 6-chloro-tryptophan, 7-chloro-tryptophan, 6-bromo-tryptophan, and 7-bromo-tryptophan. These derivatives are introduced at specific positions with corresponding bioorthogonal aminoacyl-tRNA synthetase/tRNA pairs in response to the amber codon. Following optimization, we demonstrate the robust expression of proteins containing halogenated tryptophan residues in cells with the ability to biosynthesize these tryptophan derivatives. This study establishes a versatile platform for engineering proteins with various halogenated tryptophans.

Indexed as

Genetic CodeHalogenationProtein EngineeringTryptophanAmino Acyl-tRNA SynthetasesEscherichia coliAmino Acyl-tRNA SynthetasesTryptophanBiosynthesisGenetic Code ExpansionHalogenaseHalogenated tryptophanNoncanonical amino acidProtein engineering

Identifiers

PMID38958600
PMCPMC11483216

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.