Evidence map›Paper›PMID 38958087›Full record

ArticleAutophagy2024

An APEX2-based proximity-dependent biotinylation assay with temporal specificity to study protein interactions during autophagy in the yeast

Yasmina Filali-Mouncef, Alexandre Leytens, Prado Vargas Duarte, Mattia Zampieri, Jörn Dengjel, Fulvio Reggiori

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Autophagy, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
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  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yasmina Filali-MouncefDepartment of Biomedical Sciences of Cells and Systems, University of Groningen, University Medical Center Groningen, Groningen, AV, The Netherlands.
Alexandre LeytensDepartment of Biology, University of Fribourg, Fribourg, Switzerland.
Prado Vargas DuarteDepartment of Biomedicine, Aarhus University, Aarhus C, Denmark.
Mattia ZampieriDepartment of Biomedicine, University of Basel, Basel, Switzerland.
Jörn DengjelDepartment of Biology, University of Fribourg, Fribourg, Switzerland.ORCID 0000-0002-9453-4614
Fulvio ReggioriDepartment of Biomedical Sciences of Cells and Systems, University of Groningen, University Medical Center Groningen, Groningen, AV, The Netherlands.ORCID 0000-0003-2652-2686

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Autophagosome biogenesis is a complex process orchestrated by dynamic interactions between Atg (autophagy-related) proteins and characterized by the turnover of specific cargoes, which can differ over time and depending on how autophagy is stimulated. Proteomic analyses are central to uncover protein-protein interaction networks and when combined with proximity-dependent biotinylation or proximity labeling (PL) approaches, they also permit to detect transient and weak interactions. However, current PL procedures for yeast

Indexed as

Ascorbate PeroxidasesAutophagyAutophagy-Related ProteinsBiotinylationSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsAutophagy-Related Protein 8 FamilyMembrane ProteinsProtein BindingProteomicsAscorbate PeroxidasesATG8 protein, S cerevisiaeATG9 protein, S cerevisiaeAutophagy-Related Protein 8 FamilyAutophagy-Related ProteinsMembrane ProteinsSaccharomyces cerevisiae ProteinsAtg8Atg9Atg proteinsmass spectrometryproteomicsproximity labeling

Identifiers

PMID38958087
PMCPMC11423678

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.